Helix Realtime CRISPR · Prime Simulator

Purely in-silico — stream DAG frames straight from the core engine.

Genome FASTA path (or inline FASTA) Region (optional, chr:start-end)

Mode Simulation mode

CDS (optional) CDS start (1-based) CDS end (1-based) Strand Exons (start-end, comma-separated)

Transcript config (JSON) Load transcript JSON Transcript

Schema: { transcripts: [{ id, name, strand, cds_start, cds_end, exons: [[start,end], ...] }] } (1-based)

CRISPR guide Guide sequence (5'→3') PAM pattern Max candidate sites Max depth

Prime Editing Spacer PBS RTT

pegRNA structure

Spacer pairs with the target, PBS primes, RTT is the new DNA segment that gets written into the genome.

Max depth

Status

Idle

Metrics

Nodes: 0
Edges: 0
Last frame: –

Outcome filters Intended (repaired) Indel / error No edit Other (cut/prime/flap/root)

Desired (intended/repaired) Undesired (any error/indel)

Only desired nodes

Only terminal nodes

On‑target Off‑target

Selection

Click a node to view details.

Log

Ready.