Spectronaut 21.0.260602.94842
Computer Name: HALBARAD
User Domain Name: SPECTROMASSE
User Name: voyager
Analysis Mode: UI
Analysis Type: directDIA
Analysis Date: 31-July-2026 16:42:42 UTC +02:00 


[BEGIN-SETTINGS]
Settings Used: BGS Factory Settings
   ├─ DIA Analysis\Calibration
   │  ├─ MZ Extraction Strategy:	Maximum Intensity
   │  ├─ Allow source specific iRT Calibration:	True
   │  ├─ Precision iRT:	True
   │  │  ├─ Exclude De-amidated Peptides:	True
   │  │  └─ iRT <-> RT Regression Type:	Local (Non-Linear) Regression
   │  └─ Calibration Carry-Over:	False
   ├─ DIA Analysis\Identification
   │  ├─ Precursor Qvalue Cutoff:	0.01
   │  ├─ Precursor Qvalue Cutoff (Experiment):	0.01
   │  ├─ Precursor PEP Cutoff:	0.2
   │  ├─ Protein Qvalue Cutoff (Experiment):	0.01
   │  ├─ Protein Qvalue Cutoff (Run):	0.05
   │  ├─ Protein PEP Cutoff:	0.75
   │  ├─ Single Hit Definition:	By Stripped Sequence
   │  ├─ Single Hit Protein Rule:	Stratified Single Hit Protein FDR
   │  ├─ Run-Level Protein Scoring:	All Observations
   │  ├─ Exclude Duplicate Assays:	True
   │  ├─ Exclude Predicted Fragment Scores:	False
   │  ├─ Generate Decoys:	True
   │  │  ├─ Decoy Generation Method:	Mutated
   │  │  │  └─ Preferred Fragment Source:	NN Predicted Fragments
   │  │  └─ Decoy Limit Strategy:	Dynamic
   │  │     └─ Library Size Fraction:	0.1
   │  ├─ ISF Filter Rule:	Keep "Likely In-Source Fragmented" Peptides
   │  └─ Pvalue Estimator:	Kernel Density Estimator
   ├─ DIA Analysis\Pipeline Mode
   │  ├─ Export All XICs:	False
   │  ├─ Export report in Parquet format:	False
   │  ├─ Generate SNE File:	True
   │  │  └─ Store Ion traces in SNE:	True
   │  ├─ Post Analysis Reports:	
   │  │  ├─ Binned CVs:	False
   │  │  ├─ Binned Identification:	False
   │  │  ├─ CV Density Line Chart:	False
   │  │  ├─ CVs Below X Bar Chart:	False
   │  │  ├─ Data Completeness Bar Chart:	False
   │  │  ├─ Modification Enrichment:	False
   │  │  ├─ Run Identifications Bar Chart:	False
   │  │  ├─ Scoring Histograms:	False
   │  │  └─ TIC Overlay:	False
   │  ├─ PTM Report Schema:	
   │  ├─ Report Schema:	BGS Factory Report (Normal)
   │  └─ Reporting Unit:	Across Experiment
   ├─ DIA Analysis\Post Analysis
   │  ├─ Differential Abundance Testing:	Unpaired t-test
   │  │  ├─ Assume Equal Variance:	False
   │  │  ├─ Group-Wise Testing Correction:	False
   │  │  ├─ Log2 Ratio Candidate Filter:	0.58
   │  │  └─ Confidence Candidate Filter:	Qvalue
   │  │     └─ Confidence:	0.05
   │  ├─ Differential Abundance Grouping:	Major Group (Quantification Settings)
   │  │  └─ Smallest Quantitative Unit:	Major Group (Quantification Settings)
   │  │     └─ Use All MS-Level Quantities:	False
   │  ├─ Calculate Explained TIC:	None
   │  ├─ Calculate Sample Correlation Matrix:	False
   │  ├─ Gene Ontology:	C:\Users\voyager\AppData\Roaming\Spectronaut\geneOntology\Ontologies\bgs_default_go-basic.obo
   │  └─ Hierarchical Clustering:	True
   │     ├─ Distance Metric:	Manhattan Distance
   │     ├─ Linkage Strategy:	Ward's Method
   │     ├─ Order Runs by Clustering:	True
   │     └─ Z-score Transformation:	False
   ├─ DIA Analysis\Protein Inference
   │  └─ Protein Inference Workflow:	Automatic
   │     └─ Inference Algorithm:	IDPicker
   ├─ DIA Analysis\PTM Workflow
   │  ├─ Input Normalization Strategy:	None
   │  └─ PTM Localization:	False
   ├─ DIA Analysis\Quantification
   │  ├─ Precursor Filtering:	Identified (Qvalue)
   │  │  ├─ Imputation Strategy:	None
   │  │  └─ Multi Channel Qvalue Filter:	Group Qvalue
   │  ├─ Proteotypicity Filter:	None
   │  ├─ Protein LFQ Method:	Automatic
   │  ├─ Quantity MS Level:	MS2
   │  ├─ Quantity Type:	Area
   │  ├─ Cross-Run Normalization:	True
   │  │  ├─ Normalization Filter Type:	None
   │  │  ├─ Normalization Strategy:	Automatic
   │  │  └─ Row Selection:	Automatic
   │  ├─ Perform background noise removal:	True
   │  ├─ Quantification window:	Synchronized
   │  ├─ Interference Correction:	True
   │  │  ├─ Only Identified Peptides:	True
   │  │  ├─ Exclude All Multi-Channel Interferences:	True
   │  │  ├─ MS1 Min:	2
   │  │  └─ MS2 Min:	3
   │  ├─ Major Group Quantity:	Mean peptide quantity
   │  ├─ Minor (Peptide) Grouping:	by Stripped Sequence
   │  ├─ Major (Protein) Grouping:	by Protein Group Id
   │  ├─ Major Group Top N:	True
   │  │  ├─ Max:	3
   │  │  └─ Min:	1
   │  ├─ Minor Group Quantity:	Mean precursor quantity
   │  ├─ Minor Group Top N:	True
   │  │  ├─ Max:	3
   │  │  └─ Min:	1
   │  ├─ Use Log2 Quantity Filter:	True
   │  │  └─ Minimum Log2 Precursor Quantity:	0
   │  └─ Perform IM Peak Picking for Quantification:	True
   ├─ DIA Analysis\Tolerances
   │  └─ Tolerances:	
   │     ├─ Calibration Tolerances:	
   │     │  ├─ MS1 Mass Tolerance Strategy:	Dynamic
   │     │  │  └─ Correction Factor:	1
   │     │  └─ MS2 Mass Tolerance Strategy:	Dynamic
   │     │     └─ Correction Factor:	1
   │     └─ Main Search Tolerances:	
   │        ├─ MS1 Mass Tolerance Strategy:	Relative
   │        │  └─ Tolerance (ppm):	3
   │        └─ MS2 Mass Tolerance Strategy:	Relative
   │           └─ Tolerance (ppm):	10
   ├─ DIA Analysis\Workflow
   │  ├─ Method Evaluation:	False
   │  ├─ MS2 DeMultiplexing:	Automatic
   │  ├─ Multi-Channel Workflow Definition:	From Library Annotation
   │  │  └─ Fallback Option:	Labeled
   │  ├─ Profiling Strategy:	None
   │  ├─ Run Limit for directDIA Library:	None
   │  ├─ Hybrid (DDA + DIA) Library:	False
   │  └─ Unify Peptide Peaks Strategy:	None
   ├─ DIA Analysis\XIC Extraction
   │  ├─ XIC IM Extraction Window:	Dynamic
   │  │  └─ Correction Factor:	1
   │  └─ XIC RT Extraction Window:	Dynamic
   │     └─ Correction Factor:	1
   ├─ Pulsar Search\Identification
   │  ├─ directDIA Workflow:	directDIA+ (Deep)
   │  │  ├─ IM Sampling Reduction:	7
   │  │  └─ RT Sampling Reduction:	1
   │  ├─ PTM Localization Filter:	False
   │  ├─ Grouped Peptide FDR:	None
   │  └─ Semi-Specific Pipeline:	Smart Enumeration (SN20)
   ├─ Pulsar Search\iRT Calibration
   │  ├─ Calibrate from Empirical RT:	False
   │  ├─ Auto-assign iRT source:	True
   │  ├─ iRT Reference Strategy:	Deep Learning Assisted iRT Regression
   │  ├─ Use Source Specific iRT:	Auto
   │  └─ Minimum Rsquare:	0.8
   ├─ Pulsar Search\Labeling
   │  └─ Channels:	
   │     ├─ Channel 1:	False
   │     ├─ Channel 2:	False
   │     ├─ Channel 3:	False
   │     ├─ Channel 4:	False
   │     └─ Channel 5:	False
   ├─ Pulsar Search\Modifications
   │  └─ Search Mode:	Closed Search
   │     ├─ Max Variable Modifications:	1
   │     ├─ Fixed Modifications::	Carbamidomethyl (C)
   │     └─ Variable Modifications::	Oxidation (M)
   ├─ Pulsar Search\Peptides
   │  ├─ Enzymes / Cleavage Rules:	Trypsin/P
   │  ├─ Digest Type:	Specific
   │  ├─ Decoy Generation Rule:	KR
   │  ├─ Max Peptide Length:	30
   │  ├─ Min Peptide Length:	6
   │  ├─ Missed Cleavages:	1
   │  └─ Toggle N-terminal M:	True
   ├─ Pulsar Search\Result Filters
   │  ├─ Fragment Ions:	
   │  │  ├─ Ion AA Length:	True
   │  │  │  └─ N:	3
   │  │  ├─ Ion Charge:	False
   │  │  ├─ Ion Loss Type:	True
   │  │  ├─ Ion Type:	False
   │  │  ├─ m/z :	True
   │  │  │  ├─ Max:	2000
   │  │  │  └─ Min:	150
   │  │  ├─ Overlapping between Channels:	False
   │  │  └─ Relative Intensity:	True
   │  │     └─ Min:	1
   │  └─ Precursors:	
   │     ├─ Amino Acids:	False
   │     ├─ Best N Fragments per Peptide:	True
   │     │  ├─ Max:	6
   │     │  └─ Min:	3
   │     ├─ Best N Peptides per Protein Group:	False
   │     ├─ Channel Count:	False
   │     ├─ FASTA Matched:	False
   │     ├─ Missed Cleavage:	False
   │     ├─ Modifications:	None
   │     ├─ Peptide Charge:	True
   │     │  ├─ Max Charge:	5
   │     │  └─ Min Charge:	1
   │     └─ Proteotypicity:	False
   ├─ Pulsar Search\Speed-Up
   │  ├─ MS2 Index:	Automatic
   │  └─ dia-PASEF Pre-Processing:	Automatic
   └─ Pulsar Search\Workflow
      ├─ Fragment Ion Selection Strategy:	Intensity Based
      ├─ In-Silico Generate Missing Channels:	False
      └─ Use DNN Predicted Ion Mobility:	Auto
[END-SETTINGS]

[BEGIN-SETUP]
Run: LFQ_Astral_DIA_15min_50ng_Condition_A_REP1.raw
   ├─ Vendor: Thermo
   ├─ File: LFQ_Astral_DIA_15min_50ng_Condition_A_REP1
   ├─ Path: "\\tol-brandir\Masse\Public datasets\ProteoBench\Module_quant_DIA_Astral\LFQ_Astral_DIA_15min_50ng_Condition_A_REP1.raw"
   ├─ Condition: Not Defined
   ├─ Replicate: 1
   ├─ HTRMS Version: 21.0.260602.94842
   ├─ Protein Databases Used
   │  └─ ProteoBenchFASTA_MixedSpecies_HYE
   │     ├─ Protein Entries: 31 889
   │     ├─ Original File: ProteoBenchFASTA_MixedSpecies_HYE.fasta
   │     ├─ Date Created: 24-February-2026 09:47:25 UTC +01:00 
   │     ├─ Date Modified: 31-July-2026 15:15:38 UTC +02:00 
   │     └─ Parsing Rule: UniProt FASTA
   └─ MS2 Method
      ├─ [380.4 - 382.4]
      ├─ [382.4 - 384.4]
      ├─ [384.4 - 386.4]
      ├─ [386.4 - 388.4]
      ├─ [388.4 - 390.4]
      ├─ [390.4 - 392.4]
      ├─ [392.4 - 394.4]
      ├─ [394.4 - 396.4]
      ├─ [396.4 - 398.4]
      ├─ [398.4 - 400.4]
      ├─ [400.4 - 402.4]
      ├─ [402.4 - 404.4]
      ├─ [404.4 - 406.4]
      ├─ [406.4 - 408.4]
      ├─ [408.4 - 410.4]
      ├─ [410.4 - 412.4]
      ├─ [412.4 - 414.4]
      ├─ [414.4 - 416.4]
      ├─ [416.4 - 418.4]
      ├─ [418.4 - 420.4]
      ├─ [420.4 - 422.4]
      ├─ [422.4 - 424.4]
      ├─ [424.4 - 426.4]
      ├─ [426.4 - 428.4]
      ├─ [428.4 - 430.4]
      ├─ [430.4 - 432.4]
      ├─ [432.4 - 434.4]
      ├─ [434.4 - 436.4]
      ├─ [436.4 - 438.4]
      ├─ [438.4 - 440.5]
      ├─ [440.5 - 442.5]
      ├─ [442.5 - 444.5]
      ├─ [444.5 - 446.5]
      ├─ [446.5 - 448.5]
      ├─ [448.5 - 450.5]
      ├─ [450.5 - 452.5]
      ├─ [452.5 - 454.5]
      ├─ [454.5 - 456.5]
      ├─ [456.5 - 458.5]
      ├─ [458.5 - 460.5]
      ├─ [460.5 - 462.5]
      ├─ [462.5 - 464.5]
      ├─ [464.5 - 466.5]
      ├─ [466.5 - 468.5]
      ├─ [468.5 - 470.5]
      ├─ [470.5 - 472.5]
      ├─ [472.5 - 474.5]
      ├─ [474.5 - 476.5]
      ├─ [476.5 - 478.5]
      ├─ [478.5 - 480.5]
      ├─ [480.5 - 482.5]
      ├─ [482.5 - 484.5]
      ├─ [484.5 - 486.5]
      ├─ [486.5 - 488.5]
      ├─ [488.5 - 490.5]
      ├─ [490.5 - 492.5]
      ├─ [492.5 - 494.5]
      ├─ [494.5 - 496.5]
      ├─ [496.5 - 498.5]
      ├─ [498.5 - 500.5]
      ├─ [500.5 - 502.5]
      ├─ [502.5 - 504.5]
      ├─ [504.5 - 506.5]
      ├─ [506.5 - 508.5]
      ├─ [508.5 - 510.5]
      ├─ [510.5 - 512.5]
      ├─ [512.5 - 514.5]
      ├─ [514.5 - 516.5]
      ├─ [516.5 - 518.5]
      ├─ [518.5 - 520.5]
      ├─ [520.5 - 522.5]
      ├─ [522.5 - 524.5]
      ├─ [524.5 - 526.5]
      ├─ [526.5 - 528.5]
      ├─ [528.5 - 530.5]
      ├─ [530.5 - 532.5]
      ├─ [532.5 - 534.5]
      ├─ [534.5 - 536.5]
      ├─ [536.5 - 538.5]
      ├─ [538.5 - 540.5]
      ├─ [540.5 - 542.5]
      ├─ [542.5 - 544.5]
      ├─ [544.5 - 546.5]
      ├─ [546.5 - 548.5]
      ├─ [548.5 - 550.5]
      ├─ [550.5 - 552.5]
      ├─ [552.5 - 554.5]
      ├─ [554.5 - 556.5]
      ├─ [556.5 - 558.5]
      ├─ [558.5 - 560.5]
      ├─ [560.5 - 562.5]
      ├─ [562.5 - 564.5]
      ├─ [564.5 - 566.5]
      ├─ [566.5 - 568.5]
      ├─ [568.5 - 570.5]
      ├─ [570.5 - 572.5]
      ├─ [572.5 - 574.5]
      ├─ [574.5 - 576.5]
      ├─ [576.5 - 578.5]
      ├─ [578.5 - 580.5]
      ├─ [580.5 - 582.5]
      ├─ [582.5 - 584.5]
      ├─ [584.5 - 586.5]
      ├─ [586.5 - 588.5]
      ├─ [588.5 - 590.5]
      ├─ [590.5 - 592.5]
      ├─ [592.5 - 594.5]
      ├─ [594.5 - 596.5]
      ├─ [596.5 - 598.5]
      ├─ [598.5 - 600.5]
      ├─ [600.5 - 602.5]
      ├─ [602.5 - 604.5]
      ├─ [604.5 - 606.5]
      ├─ [606.5 - 608.5]
      ├─ [608.5 - 610.5]
      ├─ [610.5 - 612.5]
      ├─ [612.5 - 614.5]
      ├─ [614.5 - 616.5]
      ├─ [616.5 - 618.5]
      ├─ [618.5 - 620.5]
      ├─ [620.5 - 622.5]
      ├─ [622.5 - 624.5]
      ├─ [624.5 - 626.5]
      ├─ [626.5 - 628.5]
      ├─ [628.5 - 630.5]
      ├─ [630.5 - 632.5]
      ├─ [632.5 - 634.5]
      ├─ [634.5 - 636.5]
      ├─ [636.5 - 638.5]
      ├─ [638.5 - 640.5]
      ├─ [640.5 - 642.5]
      ├─ [642.5 - 644.5]
      ├─ [644.5 - 646.5]
      ├─ [646.5 - 648.5]
      ├─ [648.5 - 650.5]
      ├─ [650.5 - 652.5]
      ├─ [652.5 - 654.5]
      ├─ [654.5 - 656.5]
      ├─ [656.5 - 658.5]
      ├─ [658.5 - 660.6]
      ├─ [660.6 - 662.6]
      ├─ [662.6 - 664.6]
      ├─ [664.6 - 666.6]
      ├─ [666.6 - 668.6]
      ├─ [668.6 - 670.6]
      ├─ [670.6 - 672.6]
      ├─ [672.6 - 674.6]
      ├─ [674.6 - 676.6]
      ├─ [676.6 - 678.6]
      ├─ [678.6 - 680.6]
      ├─ [680.6 - 682.6]
      ├─ [682.6 - 684.6]
      ├─ [684.6 - 686.6]
      ├─ [686.6 - 688.6]
      ├─ [688.6 - 690.6]
      ├─ [690.6 - 692.6]
      ├─ [692.6 - 694.6]
      ├─ [694.6 - 696.6]
      ├─ [696.6 - 698.6]
      ├─ [698.6 - 700.6]
      ├─ [700.6 - 702.6]
      ├─ [702.6 - 704.6]
      ├─ [704.6 - 706.6]
      ├─ [706.6 - 708.6]
      ├─ [708.6 - 710.6]
      ├─ [710.6 - 712.6]
      ├─ [712.6 - 714.6]
      ├─ [714.6 - 716.6]
      ├─ [716.6 - 718.6]
      ├─ [718.6 - 720.6]
      ├─ [720.6 - 722.6]
      ├─ [722.6 - 724.6]
      ├─ [724.6 - 726.6]
      ├─ [726.6 - 728.6]
      ├─ [728.6 - 730.6]
      ├─ [730.6 - 732.6]
      ├─ [732.6 - 734.6]
      ├─ [734.6 - 736.6]
      ├─ [736.6 - 738.6]
      ├─ [738.6 - 740.6]
      ├─ [740.6 - 742.6]
      ├─ [742.6 - 744.6]
      ├─ [744.6 - 746.6]
      ├─ [746.6 - 748.6]
      ├─ [748.6 - 750.6]
      ├─ [750.6 - 752.6]
      ├─ [752.6 - 754.6]
      ├─ [754.6 - 756.6]
      ├─ [756.6 - 758.6]
      ├─ [758.6 - 760.6]
      ├─ [760.6 - 762.6]
      ├─ [762.6 - 764.6]
      ├─ [764.6 - 766.6]
      ├─ [766.6 - 768.6]
      ├─ [768.6 - 770.6]
      ├─ [770.6 - 772.6]
      ├─ [772.6 - 774.6]
      ├─ [774.6 - 776.6]
      ├─ [776.6 - 778.6]
      ├─ [778.6 - 780.6]
      ├─ [780.6 - 782.6]
      ├─ [782.6 - 784.6]
      ├─ [784.6 - 786.6]
      ├─ [786.6 - 788.6]
      ├─ [788.6 - 790.6]
      ├─ [790.6 - 792.6]
      ├─ [792.6 - 794.6]
      ├─ [794.6 - 796.6]
      ├─ [796.6 - 798.6]
      ├─ [798.6 - 800.6]
      ├─ [800.6 - 802.6]
      ├─ [802.6 - 804.6]
      ├─ [804.6 - 806.6]
      ├─ [806.6 - 808.6]
      ├─ [808.6 - 810.6]
      ├─ [810.6 - 812.6]
      ├─ [812.6 - 814.6]
      ├─ [814.6 - 816.6]
      ├─ [816.6 - 818.6]
      ├─ [818.6 - 820.6]
      ├─ [820.6 - 822.6]
      ├─ [822.6 - 824.6]
      ├─ [824.6 - 826.6]
      ├─ [826.6 - 828.6]
      ├─ [828.6 - 830.6]
      ├─ [830.6 - 832.6]
      ├─ [832.6 - 834.6]
      ├─ [834.6 - 836.6]
      ├─ [836.6 - 838.6]
      ├─ [838.6 - 840.6]
      ├─ [840.6 - 842.6]
      ├─ [842.6 - 844.6]
      ├─ [844.6 - 846.6]
      ├─ [846.6 - 848.6]
      ├─ [848.6 - 850.6]
      ├─ [850.6 - 852.6]
      ├─ [852.6 - 854.6]
      ├─ [854.6 - 856.6]
      ├─ [856.6 - 858.6]
      ├─ [858.6 - 860.6]
      ├─ [860.6 - 862.6]
      ├─ [862.6 - 864.6]
      ├─ [864.6 - 866.6]
      ├─ [866.6 - 868.6]
      ├─ [868.6 - 870.6]
      ├─ [870.6 - 872.6]
      ├─ [872.6 - 874.6]
      ├─ [874.6 - 876.6]
      ├─ [876.6 - 878.6]
      ├─ [878.6 - 880.7]
      ├─ [880.7 - 882.7]
      ├─ [882.7 - 884.7]
      ├─ [884.7 - 886.7]
      ├─ [886.7 - 888.7]
      ├─ [888.7 - 890.7]
      ├─ [890.7 - 892.7]
      ├─ [892.7 - 894.7]
      ├─ [894.7 - 896.7]
      ├─ [896.7 - 898.7]
      ├─ [898.7 - 900.7]
      ├─ [900.7 - 902.7]
      ├─ [902.7 - 904.7]
      ├─ [904.7 - 906.7]
      ├─ [906.7 - 908.7]
      ├─ [908.7 - 910.7]
      ├─ [910.7 - 912.7]
      ├─ [912.7 - 914.7]
      ├─ [914.7 - 916.7]
      ├─ [916.7 - 918.7]
      ├─ [918.7 - 920.7]
      ├─ [920.7 - 922.7]
      ├─ [922.7 - 924.7]
      ├─ [924.7 - 926.7]
      ├─ [926.7 - 928.7]
      ├─ [928.7 - 930.7]
      ├─ [930.7 - 932.7]
      ├─ [932.7 - 934.7]
      ├─ [934.7 - 936.7]
      ├─ [936.7 - 938.7]
      ├─ [938.7 - 940.7]
      ├─ [940.7 - 942.7]
      ├─ [942.7 - 944.7]
      ├─ [944.7 - 946.7]
      ├─ [946.7 - 948.7]
      ├─ [948.7 - 950.7]
      ├─ [950.7 - 952.7]
      ├─ [952.7 - 954.7]
      ├─ [954.7 - 956.7]
      ├─ [956.7 - 958.7]
      ├─ [958.7 - 960.7]
      ├─ [960.7 - 962.7]
      ├─ [962.7 - 964.7]
      ├─ [964.7 - 966.7]
      ├─ [966.7 - 968.7]
      ├─ [968.7 - 970.7]
      ├─ [970.7 - 972.7]
      ├─ [972.7 - 974.7]
      ├─ [974.7 - 976.7]
      ├─ [976.7 - 978.7]
      └─ [978.7 - 980.7]

Run: LFQ_Astral_DIA_15min_50ng_Condition_A_REP2.raw
   ├─ Vendor: Thermo
   ├─ File: LFQ_Astral_DIA_15min_50ng_Condition_A_REP2
   ├─ Path: "\\tol-brandir\Masse\Public datasets\ProteoBench\Module_quant_DIA_Astral\LFQ_Astral_DIA_15min_50ng_Condition_A_REP2.raw"
   ├─ Condition: Not Defined
   ├─ Replicate: 2
   ├─ HTRMS Version: 21.0.260602.94842
   ├─ Protein Databases Used
   │  └─ ProteoBenchFASTA_MixedSpecies_HYE
   │     ├─ Protein Entries: 31 889
   │     ├─ Original File: ProteoBenchFASTA_MixedSpecies_HYE.fasta
   │     ├─ Date Created: 24-February-2026 09:47:25 UTC +01:00 
   │     ├─ Date Modified: 31-July-2026 15:15:38 UTC +02:00 
   │     └─ Parsing Rule: UniProt FASTA
   └─ MS2 Method
      ├─ [380.4 - 382.4]
      ├─ [382.4 - 384.4]
      ├─ [384.4 - 386.4]
      ├─ [386.4 - 388.4]
      ├─ [388.4 - 390.4]
      ├─ [390.4 - 392.4]
      ├─ [392.4 - 394.4]
      ├─ [394.4 - 396.4]
      ├─ [396.4 - 398.4]
      ├─ [398.4 - 400.4]
      ├─ [400.4 - 402.4]
      ├─ [402.4 - 404.4]
      ├─ [404.4 - 406.4]
      ├─ [406.4 - 408.4]
      ├─ [408.4 - 410.4]
      ├─ [410.4 - 412.4]
      ├─ [412.4 - 414.4]
      ├─ [414.4 - 416.4]
      ├─ [416.4 - 418.4]
      ├─ [418.4 - 420.4]
      ├─ [420.4 - 422.4]
      ├─ [422.4 - 424.4]
      ├─ [424.4 - 426.4]
      ├─ [426.4 - 428.4]
      ├─ [428.4 - 430.4]
      ├─ [430.4 - 432.4]
      ├─ [432.4 - 434.4]
      ├─ [434.4 - 436.4]
      ├─ [436.4 - 438.4]
      ├─ [438.4 - 440.5]
      ├─ [440.5 - 442.5]
      ├─ [442.5 - 444.5]
      ├─ [444.5 - 446.5]
      ├─ [446.5 - 448.5]
      ├─ [448.5 - 450.5]
      ├─ [450.5 - 452.5]
      ├─ [452.5 - 454.5]
      ├─ [454.5 - 456.5]
      ├─ [456.5 - 458.5]
      ├─ [458.5 - 460.5]
      ├─ [460.5 - 462.5]
      ├─ [462.5 - 464.5]
      ├─ [464.5 - 466.5]
      ├─ [466.5 - 468.5]
      ├─ [468.5 - 470.5]
      ├─ [470.5 - 472.5]
      ├─ [472.5 - 474.5]
      ├─ [474.5 - 476.5]
      ├─ [476.5 - 478.5]
      ├─ [478.5 - 480.5]
      ├─ [480.5 - 482.5]
      ├─ [482.5 - 484.5]
      ├─ [484.5 - 486.5]
      ├─ [486.5 - 488.5]
      ├─ [488.5 - 490.5]
      ├─ [490.5 - 492.5]
      ├─ [492.5 - 494.5]
      ├─ [494.5 - 496.5]
      ├─ [496.5 - 498.5]
      ├─ [498.5 - 500.5]
      ├─ [500.5 - 502.5]
      ├─ [502.5 - 504.5]
      ├─ [504.5 - 506.5]
      ├─ [506.5 - 508.5]
      ├─ [508.5 - 510.5]
      ├─ [510.5 - 512.5]
      ├─ [512.5 - 514.5]
      ├─ [514.5 - 516.5]
      ├─ [516.5 - 518.5]
      ├─ [518.5 - 520.5]
      ├─ [520.5 - 522.5]
      ├─ [522.5 - 524.5]
      ├─ [524.5 - 526.5]
      ├─ [526.5 - 528.5]
      ├─ [528.5 - 530.5]
      ├─ [530.5 - 532.5]
      ├─ [532.5 - 534.5]
      ├─ [534.5 - 536.5]
      ├─ [536.5 - 538.5]
      ├─ [538.5 - 540.5]
      ├─ [540.5 - 542.5]
      ├─ [542.5 - 544.5]
      ├─ [544.5 - 546.5]
      ├─ [546.5 - 548.5]
      ├─ [548.5 - 550.5]
      ├─ [550.5 - 552.5]
      ├─ [552.5 - 554.5]
      ├─ [554.5 - 556.5]
      ├─ [556.5 - 558.5]
      ├─ [558.5 - 560.5]
      ├─ [560.5 - 562.5]
      ├─ [562.5 - 564.5]
      ├─ [564.5 - 566.5]
      ├─ [566.5 - 568.5]
      ├─ [568.5 - 570.5]
      ├─ [570.5 - 572.5]
      ├─ [572.5 - 574.5]
      ├─ [574.5 - 576.5]
      ├─ [576.5 - 578.5]
      ├─ [578.5 - 580.5]
      ├─ [580.5 - 582.5]
      ├─ [582.5 - 584.5]
      ├─ [584.5 - 586.5]
      ├─ [586.5 - 588.5]
      ├─ [588.5 - 590.5]
      ├─ [590.5 - 592.5]
      ├─ [592.5 - 594.5]
      ├─ [594.5 - 596.5]
      ├─ [596.5 - 598.5]
      ├─ [598.5 - 600.5]
      ├─ [600.5 - 602.5]
      ├─ [602.5 - 604.5]
      ├─ [604.5 - 606.5]
      ├─ [606.5 - 608.5]
      ├─ [608.5 - 610.5]
      ├─ [610.5 - 612.5]
      ├─ [612.5 - 614.5]
      ├─ [614.5 - 616.5]
      ├─ [616.5 - 618.5]
      ├─ [618.5 - 620.5]
      ├─ [620.5 - 622.5]
      ├─ [622.5 - 624.5]
      ├─ [624.5 - 626.5]
      ├─ [626.5 - 628.5]
      ├─ [628.5 - 630.5]
      ├─ [630.5 - 632.5]
      ├─ [632.5 - 634.5]
      ├─ [634.5 - 636.5]
      ├─ [636.5 - 638.5]
      ├─ [638.5 - 640.5]
      ├─ [640.5 - 642.5]
      ├─ [642.5 - 644.5]
      ├─ [644.5 - 646.5]
      ├─ [646.5 - 648.5]
      ├─ [648.5 - 650.5]
      ├─ [650.5 - 652.5]
      ├─ [652.5 - 654.5]
      ├─ [654.5 - 656.5]
      ├─ [656.5 - 658.5]
      ├─ [658.5 - 660.6]
      ├─ [660.6 - 662.6]
      ├─ [662.6 - 664.6]
      ├─ [664.6 - 666.6]
      ├─ [666.6 - 668.6]
      ├─ [668.6 - 670.6]
      ├─ [670.6 - 672.6]
      ├─ [672.6 - 674.6]
      ├─ [674.6 - 676.6]
      ├─ [676.6 - 678.6]
      ├─ [678.6 - 680.6]
      ├─ [680.6 - 682.6]
      ├─ [682.6 - 684.6]
      ├─ [684.6 - 686.6]
      ├─ [686.6 - 688.6]
      ├─ [688.6 - 690.6]
      ├─ [690.6 - 692.6]
      ├─ [692.6 - 694.6]
      ├─ [694.6 - 696.6]
      ├─ [696.6 - 698.6]
      ├─ [698.6 - 700.6]
      ├─ [700.6 - 702.6]
      ├─ [702.6 - 704.6]
      ├─ [704.6 - 706.6]
      ├─ [706.6 - 708.6]
      ├─ [708.6 - 710.6]
      ├─ [710.6 - 712.6]
      ├─ [712.6 - 714.6]
      ├─ [714.6 - 716.6]
      ├─ [716.6 - 718.6]
      ├─ [718.6 - 720.6]
      ├─ [720.6 - 722.6]
      ├─ [722.6 - 724.6]
      ├─ [724.6 - 726.6]
      ├─ [726.6 - 728.6]
      ├─ [728.6 - 730.6]
      ├─ [730.6 - 732.6]
      ├─ [732.6 - 734.6]
      ├─ [734.6 - 736.6]
      ├─ [736.6 - 738.6]
      ├─ [738.6 - 740.6]
      ├─ [740.6 - 742.6]
      ├─ [742.6 - 744.6]
      ├─ [744.6 - 746.6]
      ├─ [746.6 - 748.6]
      ├─ [748.6 - 750.6]
      ├─ [750.6 - 752.6]
      ├─ [752.6 - 754.6]
      ├─ [754.6 - 756.6]
      ├─ [756.6 - 758.6]
      ├─ [758.6 - 760.6]
      ├─ [760.6 - 762.6]
      ├─ [762.6 - 764.6]
      ├─ [764.6 - 766.6]
      ├─ [766.6 - 768.6]
      ├─ [768.6 - 770.6]
      ├─ [770.6 - 772.6]
      ├─ [772.6 - 774.6]
      ├─ [774.6 - 776.6]
      ├─ [776.6 - 778.6]
      ├─ [778.6 - 780.6]
      ├─ [780.6 - 782.6]
      ├─ [782.6 - 784.6]
      ├─ [784.6 - 786.6]
      ├─ [786.6 - 788.6]
      ├─ [788.6 - 790.6]
      ├─ [790.6 - 792.6]
      ├─ [792.6 - 794.6]
      ├─ [794.6 - 796.6]
      ├─ [796.6 - 798.6]
      ├─ [798.6 - 800.6]
      ├─ [800.6 - 802.6]
      ├─ [802.6 - 804.6]
      ├─ [804.6 - 806.6]
      ├─ [806.6 - 808.6]
      ├─ [808.6 - 810.6]
      ├─ [810.6 - 812.6]
      ├─ [812.6 - 814.6]
      ├─ [814.6 - 816.6]
      ├─ [816.6 - 818.6]
      ├─ [818.6 - 820.6]
      ├─ [820.6 - 822.6]
      ├─ [822.6 - 824.6]
      ├─ [824.6 - 826.6]
      ├─ [826.6 - 828.6]
      ├─ [828.6 - 830.6]
      ├─ [830.6 - 832.6]
      ├─ [832.6 - 834.6]
      ├─ [834.6 - 836.6]
      ├─ [836.6 - 838.6]
      ├─ [838.6 - 840.6]
      ├─ [840.6 - 842.6]
      ├─ [842.6 - 844.6]
      ├─ [844.6 - 846.6]
      ├─ [846.6 - 848.6]
      ├─ [848.6 - 850.6]
      ├─ [850.6 - 852.6]
      ├─ [852.6 - 854.6]
      ├─ [854.6 - 856.6]
      ├─ [856.6 - 858.6]
      ├─ [858.6 - 860.6]
      ├─ [860.6 - 862.6]
      ├─ [862.6 - 864.6]
      ├─ [864.6 - 866.6]
      ├─ [866.6 - 868.6]
      ├─ [868.6 - 870.6]
      ├─ [870.6 - 872.6]
      ├─ [872.6 - 874.6]
      ├─ [874.6 - 876.6]
      ├─ [876.6 - 878.6]
      ├─ [878.6 - 880.7]
      ├─ [880.7 - 882.7]
      ├─ [882.7 - 884.7]
      ├─ [884.7 - 886.7]
      ├─ [886.7 - 888.7]
      ├─ [888.7 - 890.7]
      ├─ [890.7 - 892.7]
      ├─ [892.7 - 894.7]
      ├─ [894.7 - 896.7]
      ├─ [896.7 - 898.7]
      ├─ [898.7 - 900.7]
      ├─ [900.7 - 902.7]
      ├─ [902.7 - 904.7]
      ├─ [904.7 - 906.7]
      ├─ [906.7 - 908.7]
      ├─ [908.7 - 910.7]
      ├─ [910.7 - 912.7]
      ├─ [912.7 - 914.7]
      ├─ [914.7 - 916.7]
      ├─ [916.7 - 918.7]
      ├─ [918.7 - 920.7]
      ├─ [920.7 - 922.7]
      ├─ [922.7 - 924.7]
      ├─ [924.7 - 926.7]
      ├─ [926.7 - 928.7]
      ├─ [928.7 - 930.7]
      ├─ [930.7 - 932.7]
      ├─ [932.7 - 934.7]
      ├─ [934.7 - 936.7]
      ├─ [936.7 - 938.7]
      ├─ [938.7 - 940.7]
      ├─ [940.7 - 942.7]
      ├─ [942.7 - 944.7]
      ├─ [944.7 - 946.7]
      ├─ [946.7 - 948.7]
      ├─ [948.7 - 950.7]
      ├─ [950.7 - 952.7]
      ├─ [952.7 - 954.7]
      ├─ [954.7 - 956.7]
      ├─ [956.7 - 958.7]
      ├─ [958.7 - 960.7]
      ├─ [960.7 - 962.7]
      ├─ [962.7 - 964.7]
      ├─ [964.7 - 966.7]
      ├─ [966.7 - 968.7]
      ├─ [968.7 - 970.7]
      ├─ [970.7 - 972.7]
      ├─ [972.7 - 974.7]
      ├─ [974.7 - 976.7]
      ├─ [976.7 - 978.7]
      └─ [978.7 - 980.7]

Run: LFQ_Astral_DIA_15min_50ng_Condition_A_REP3.raw
   ├─ Vendor: Thermo
   ├─ File: LFQ_Astral_DIA_15min_50ng_Condition_A_REP3
   ├─ Path: "\\tol-brandir\Masse\Public datasets\ProteoBench\Module_quant_DIA_Astral\LFQ_Astral_DIA_15min_50ng_Condition_A_REP3.raw"
   ├─ Condition: Not Defined
   ├─ Replicate: 3
   ├─ HTRMS Version: 21.0.260602.94842
   ├─ Protein Databases Used
   │  └─ ProteoBenchFASTA_MixedSpecies_HYE
   │     ├─ Protein Entries: 31 889
   │     ├─ Original File: ProteoBenchFASTA_MixedSpecies_HYE.fasta
   │     ├─ Date Created: 24-February-2026 09:47:25 UTC +01:00 
   │     ├─ Date Modified: 31-July-2026 15:15:38 UTC +02:00 
   │     └─ Parsing Rule: UniProt FASTA
   └─ MS2 Method
      ├─ [380.4 - 382.4]
      ├─ [382.4 - 384.4]
      ├─ [384.4 - 386.4]
      ├─ [386.4 - 388.4]
      ├─ [388.4 - 390.4]
      ├─ [390.4 - 392.4]
      ├─ [392.4 - 394.4]
      ├─ [394.4 - 396.4]
      ├─ [396.4 - 398.4]
      ├─ [398.4 - 400.4]
      ├─ [400.4 - 402.4]
      ├─ [402.4 - 404.4]
      ├─ [404.4 - 406.4]
      ├─ [406.4 - 408.4]
      ├─ [408.4 - 410.4]
      ├─ [410.4 - 412.4]
      ├─ [412.4 - 414.4]
      ├─ [414.4 - 416.4]
      ├─ [416.4 - 418.4]
      ├─ [418.4 - 420.4]
      ├─ [420.4 - 422.4]
      ├─ [422.4 - 424.4]
      ├─ [424.4 - 426.4]
      ├─ [426.4 - 428.4]
      ├─ [428.4 - 430.4]
      ├─ [430.4 - 432.4]
      ├─ [432.4 - 434.4]
      ├─ [434.4 - 436.4]
      ├─ [436.4 - 438.4]
      ├─ [438.4 - 440.5]
      ├─ [440.5 - 442.5]
      ├─ [442.5 - 444.5]
      ├─ [444.5 - 446.5]
      ├─ [446.5 - 448.5]
      ├─ [448.5 - 450.5]
      ├─ [450.5 - 452.5]
      ├─ [452.5 - 454.5]
      ├─ [454.5 - 456.5]
      ├─ [456.5 - 458.5]
      ├─ [458.5 - 460.5]
      ├─ [460.5 - 462.5]
      ├─ [462.5 - 464.5]
      ├─ [464.5 - 466.5]
      ├─ [466.5 - 468.5]
      ├─ [468.5 - 470.5]
      ├─ [470.5 - 472.5]
      ├─ [472.5 - 474.5]
      ├─ [474.5 - 476.5]
      ├─ [476.5 - 478.5]
      ├─ [478.5 - 480.5]
      ├─ [480.5 - 482.5]
      ├─ [482.5 - 484.5]
      ├─ [484.5 - 486.5]
      ├─ [486.5 - 488.5]
      ├─ [488.5 - 490.5]
      ├─ [490.5 - 492.5]
      ├─ [492.5 - 494.5]
      ├─ [494.5 - 496.5]
      ├─ [496.5 - 498.5]
      ├─ [498.5 - 500.5]
      ├─ [500.5 - 502.5]
      ├─ [502.5 - 504.5]
      ├─ [504.5 - 506.5]
      ├─ [506.5 - 508.5]
      ├─ [508.5 - 510.5]
      ├─ [510.5 - 512.5]
      ├─ [512.5 - 514.5]
      ├─ [514.5 - 516.5]
      ├─ [516.5 - 518.5]
      ├─ [518.5 - 520.5]
      ├─ [520.5 - 522.5]
      ├─ [522.5 - 524.5]
      ├─ [524.5 - 526.5]
      ├─ [526.5 - 528.5]
      ├─ [528.5 - 530.5]
      ├─ [530.5 - 532.5]
      ├─ [532.5 - 534.5]
      ├─ [534.5 - 536.5]
      ├─ [536.5 - 538.5]
      ├─ [538.5 - 540.5]
      ├─ [540.5 - 542.5]
      ├─ [542.5 - 544.5]
      ├─ [544.5 - 546.5]
      ├─ [546.5 - 548.5]
      ├─ [548.5 - 550.5]
      ├─ [550.5 - 552.5]
      ├─ [552.5 - 554.5]
      ├─ [554.5 - 556.5]
      ├─ [556.5 - 558.5]
      ├─ [558.5 - 560.5]
      ├─ [560.5 - 562.5]
      ├─ [562.5 - 564.5]
      ├─ [564.5 - 566.5]
      ├─ [566.5 - 568.5]
      ├─ [568.5 - 570.5]
      ├─ [570.5 - 572.5]
      ├─ [572.5 - 574.5]
      ├─ [574.5 - 576.5]
      ├─ [576.5 - 578.5]
      ├─ [578.5 - 580.5]
      ├─ [580.5 - 582.5]
      ├─ [582.5 - 584.5]
      ├─ [584.5 - 586.5]
      ├─ [586.5 - 588.5]
      ├─ [588.5 - 590.5]
      ├─ [590.5 - 592.5]
      ├─ [592.5 - 594.5]
      ├─ [594.5 - 596.5]
      ├─ [596.5 - 598.5]
      ├─ [598.5 - 600.5]
      ├─ [600.5 - 602.5]
      ├─ [602.5 - 604.5]
      ├─ [604.5 - 606.5]
      ├─ [606.5 - 608.5]
      ├─ [608.5 - 610.5]
      ├─ [610.5 - 612.5]
      ├─ [612.5 - 614.5]
      ├─ [614.5 - 616.5]
      ├─ [616.5 - 618.5]
      ├─ [618.5 - 620.5]
      ├─ [620.5 - 622.5]
      ├─ [622.5 - 624.5]
      ├─ [624.5 - 626.5]
      ├─ [626.5 - 628.5]
      ├─ [628.5 - 630.5]
      ├─ [630.5 - 632.5]
      ├─ [632.5 - 634.5]
      ├─ [634.5 - 636.5]
      ├─ [636.5 - 638.5]
      ├─ [638.5 - 640.5]
      ├─ [640.5 - 642.5]
      ├─ [642.5 - 644.5]
      ├─ [644.5 - 646.5]
      ├─ [646.5 - 648.5]
      ├─ [648.5 - 650.5]
      ├─ [650.5 - 652.5]
      ├─ [652.5 - 654.5]
      ├─ [654.5 - 656.5]
      ├─ [656.5 - 658.5]
      ├─ [658.5 - 660.6]
      ├─ [660.6 - 662.6]
      ├─ [662.6 - 664.6]
      ├─ [664.6 - 666.6]
      ├─ [666.6 - 668.6]
      ├─ [668.6 - 670.6]
      ├─ [670.6 - 672.6]
      ├─ [672.6 - 674.6]
      ├─ [674.6 - 676.6]
      ├─ [676.6 - 678.6]
      ├─ [678.6 - 680.6]
      ├─ [680.6 - 682.6]
      ├─ [682.6 - 684.6]
      ├─ [684.6 - 686.6]
      ├─ [686.6 - 688.6]
      ├─ [688.6 - 690.6]
      ├─ [690.6 - 692.6]
      ├─ [692.6 - 694.6]
      ├─ [694.6 - 696.6]
      ├─ [696.6 - 698.6]
      ├─ [698.6 - 700.6]
      ├─ [700.6 - 702.6]
      ├─ [702.6 - 704.6]
      ├─ [704.6 - 706.6]
      ├─ [706.6 - 708.6]
      ├─ [708.6 - 710.6]
      ├─ [710.6 - 712.6]
      ├─ [712.6 - 714.6]
      ├─ [714.6 - 716.6]
      ├─ [716.6 - 718.6]
      ├─ [718.6 - 720.6]
      ├─ [720.6 - 722.6]
      ├─ [722.6 - 724.6]
      ├─ [724.6 - 726.6]
      ├─ [726.6 - 728.6]
      ├─ [728.6 - 730.6]
      ├─ [730.6 - 732.6]
      ├─ [732.6 - 734.6]
      ├─ [734.6 - 736.6]
      ├─ [736.6 - 738.6]
      ├─ [738.6 - 740.6]
      ├─ [740.6 - 742.6]
      ├─ [742.6 - 744.6]
      ├─ [744.6 - 746.6]
      ├─ [746.6 - 748.6]
      ├─ [748.6 - 750.6]
      ├─ [750.6 - 752.6]
      ├─ [752.6 - 754.6]
      ├─ [754.6 - 756.6]
      ├─ [756.6 - 758.6]
      ├─ [758.6 - 760.6]
      ├─ [760.6 - 762.6]
      ├─ [762.6 - 764.6]
      ├─ [764.6 - 766.6]
      ├─ [766.6 - 768.6]
      ├─ [768.6 - 770.6]
      ├─ [770.6 - 772.6]
      ├─ [772.6 - 774.6]
      ├─ [774.6 - 776.6]
      ├─ [776.6 - 778.6]
      ├─ [778.6 - 780.6]
      ├─ [780.6 - 782.6]
      ├─ [782.6 - 784.6]
      ├─ [784.6 - 786.6]
      ├─ [786.6 - 788.6]
      ├─ [788.6 - 790.6]
      ├─ [790.6 - 792.6]
      ├─ [792.6 - 794.6]
      ├─ [794.6 - 796.6]
      ├─ [796.6 - 798.6]
      ├─ [798.6 - 800.6]
      ├─ [800.6 - 802.6]
      ├─ [802.6 - 804.6]
      ├─ [804.6 - 806.6]
      ├─ [806.6 - 808.6]
      ├─ [808.6 - 810.6]
      ├─ [810.6 - 812.6]
      ├─ [812.6 - 814.6]
      ├─ [814.6 - 816.6]
      ├─ [816.6 - 818.6]
      ├─ [818.6 - 820.6]
      ├─ [820.6 - 822.6]
      ├─ [822.6 - 824.6]
      ├─ [824.6 - 826.6]
      ├─ [826.6 - 828.6]
      ├─ [828.6 - 830.6]
      ├─ [830.6 - 832.6]
      ├─ [832.6 - 834.6]
      ├─ [834.6 - 836.6]
      ├─ [836.6 - 838.6]
      ├─ [838.6 - 840.6]
      ├─ [840.6 - 842.6]
      ├─ [842.6 - 844.6]
      ├─ [844.6 - 846.6]
      ├─ [846.6 - 848.6]
      ├─ [848.6 - 850.6]
      ├─ [850.6 - 852.6]
      ├─ [852.6 - 854.6]
      ├─ [854.6 - 856.6]
      ├─ [856.6 - 858.6]
      ├─ [858.6 - 860.6]
      ├─ [860.6 - 862.6]
      ├─ [862.6 - 864.6]
      ├─ [864.6 - 866.6]
      ├─ [866.6 - 868.6]
      ├─ [868.6 - 870.6]
      ├─ [870.6 - 872.6]
      ├─ [872.6 - 874.6]
      ├─ [874.6 - 876.6]
      ├─ [876.6 - 878.6]
      ├─ [878.6 - 880.7]
      ├─ [880.7 - 882.7]
      ├─ [882.7 - 884.7]
      ├─ [884.7 - 886.7]
      ├─ [886.7 - 888.7]
      ├─ [888.7 - 890.7]
      ├─ [890.7 - 892.7]
      ├─ [892.7 - 894.7]
      ├─ [894.7 - 896.7]
      ├─ [896.7 - 898.7]
      ├─ [898.7 - 900.7]
      ├─ [900.7 - 902.7]
      ├─ [902.7 - 904.7]
      ├─ [904.7 - 906.7]
      ├─ [906.7 - 908.7]
      ├─ [908.7 - 910.7]
      ├─ [910.7 - 912.7]
      ├─ [912.7 - 914.7]
      ├─ [914.7 - 916.7]
      ├─ [916.7 - 918.7]
      ├─ [918.7 - 920.7]
      ├─ [920.7 - 922.7]
      ├─ [922.7 - 924.7]
      ├─ [924.7 - 926.7]
      ├─ [926.7 - 928.7]
      ├─ [928.7 - 930.7]
      ├─ [930.7 - 932.7]
      ├─ [932.7 - 934.7]
      ├─ [934.7 - 936.7]
      ├─ [936.7 - 938.7]
      ├─ [938.7 - 940.7]
      ├─ [940.7 - 942.7]
      ├─ [942.7 - 944.7]
      ├─ [944.7 - 946.7]
      ├─ [946.7 - 948.7]
      ├─ [948.7 - 950.7]
      ├─ [950.7 - 952.7]
      ├─ [952.7 - 954.7]
      ├─ [954.7 - 956.7]
      ├─ [956.7 - 958.7]
      ├─ [958.7 - 960.7]
      ├─ [960.7 - 962.7]
      ├─ [962.7 - 964.7]
      ├─ [964.7 - 966.7]
      ├─ [966.7 - 968.7]
      ├─ [968.7 - 970.7]
      ├─ [970.7 - 972.7]
      ├─ [972.7 - 974.7]
      ├─ [974.7 - 976.7]
      ├─ [976.7 - 978.7]
      └─ [978.7 - 980.7]

Run: LFQ_Astral_DIA_15min_50ng_Condition_B_REP1.raw
   ├─ Vendor: Thermo
   ├─ File: LFQ_Astral_DIA_15min_50ng_Condition_B_REP1
   ├─ Path: "\\tol-brandir\Masse\Public datasets\ProteoBench\Module_quant_DIA_Astral\LFQ_Astral_DIA_15min_50ng_Condition_B_REP1.raw"
   ├─ Condition: Not Defined
   ├─ Replicate: 4
   ├─ HTRMS Version: 21.0.260602.94842
   ├─ Protein Databases Used
   │  └─ ProteoBenchFASTA_MixedSpecies_HYE
   │     ├─ Protein Entries: 31 889
   │     ├─ Original File: ProteoBenchFASTA_MixedSpecies_HYE.fasta
   │     ├─ Date Created: 24-February-2026 09:47:25 UTC +01:00 
   │     ├─ Date Modified: 31-July-2026 15:15:38 UTC +02:00 
   │     └─ Parsing Rule: UniProt FASTA
   └─ MS2 Method
      ├─ [380.4 - 382.4]
      ├─ [382.4 - 384.4]
      ├─ [384.4 - 386.4]
      ├─ [386.4 - 388.4]
      ├─ [388.4 - 390.4]
      ├─ [390.4 - 392.4]
      ├─ [392.4 - 394.4]
      ├─ [394.4 - 396.4]
      ├─ [396.4 - 398.4]
      ├─ [398.4 - 400.4]
      ├─ [400.4 - 402.4]
      ├─ [402.4 - 404.4]
      ├─ [404.4 - 406.4]
      ├─ [406.4 - 408.4]
      ├─ [408.4 - 410.4]
      ├─ [410.4 - 412.4]
      ├─ [412.4 - 414.4]
      ├─ [414.4 - 416.4]
      ├─ [416.4 - 418.4]
      ├─ [418.4 - 420.4]
      ├─ [420.4 - 422.4]
      ├─ [422.4 - 424.4]
      ├─ [424.4 - 426.4]
      ├─ [426.4 - 428.4]
      ├─ [428.4 - 430.4]
      ├─ [430.4 - 432.4]
      ├─ [432.4 - 434.4]
      ├─ [434.4 - 436.4]
      ├─ [436.4 - 438.4]
      ├─ [438.4 - 440.5]
      ├─ [440.5 - 442.5]
      ├─ [442.5 - 444.5]
      ├─ [444.5 - 446.5]
      ├─ [446.5 - 448.5]
      ├─ [448.5 - 450.5]
      ├─ [450.5 - 452.5]
      ├─ [452.5 - 454.5]
      ├─ [454.5 - 456.5]
      ├─ [456.5 - 458.5]
      ├─ [458.5 - 460.5]
      ├─ [460.5 - 462.5]
      ├─ [462.5 - 464.5]
      ├─ [464.5 - 466.5]
      ├─ [466.5 - 468.5]
      ├─ [468.5 - 470.5]
      ├─ [470.5 - 472.5]
      ├─ [472.5 - 474.5]
      ├─ [474.5 - 476.5]
      ├─ [476.5 - 478.5]
      ├─ [478.5 - 480.5]
      ├─ [480.5 - 482.5]
      ├─ [482.5 - 484.5]
      ├─ [484.5 - 486.5]
      ├─ [486.5 - 488.5]
      ├─ [488.5 - 490.5]
      ├─ [490.5 - 492.5]
      ├─ [492.5 - 494.5]
      ├─ [494.5 - 496.5]
      ├─ [496.5 - 498.5]
      ├─ [498.5 - 500.5]
      ├─ [500.5 - 502.5]
      ├─ [502.5 - 504.5]
      ├─ [504.5 - 506.5]
      ├─ [506.5 - 508.5]
      ├─ [508.5 - 510.5]
      ├─ [510.5 - 512.5]
      ├─ [512.5 - 514.5]
      ├─ [514.5 - 516.5]
      ├─ [516.5 - 518.5]
      ├─ [518.5 - 520.5]
      ├─ [520.5 - 522.5]
      ├─ [522.5 - 524.5]
      ├─ [524.5 - 526.5]
      ├─ [526.5 - 528.5]
      ├─ [528.5 - 530.5]
      ├─ [530.5 - 532.5]
      ├─ [532.5 - 534.5]
      ├─ [534.5 - 536.5]
      ├─ [536.5 - 538.5]
      ├─ [538.5 - 540.5]
      ├─ [540.5 - 542.5]
      ├─ [542.5 - 544.5]
      ├─ [544.5 - 546.5]
      ├─ [546.5 - 548.5]
      ├─ [548.5 - 550.5]
      ├─ [550.5 - 552.5]
      ├─ [552.5 - 554.5]
      ├─ [554.5 - 556.5]
      ├─ [556.5 - 558.5]
      ├─ [558.5 - 560.5]
      ├─ [560.5 - 562.5]
      ├─ [562.5 - 564.5]
      ├─ [564.5 - 566.5]
      ├─ [566.5 - 568.5]
      ├─ [568.5 - 570.5]
      ├─ [570.5 - 572.5]
      ├─ [572.5 - 574.5]
      ├─ [574.5 - 576.5]
      ├─ [576.5 - 578.5]
      ├─ [578.5 - 580.5]
      ├─ [580.5 - 582.5]
      ├─ [582.5 - 584.5]
      ├─ [584.5 - 586.5]
      ├─ [586.5 - 588.5]
      ├─ [588.5 - 590.5]
      ├─ [590.5 - 592.5]
      ├─ [592.5 - 594.5]
      ├─ [594.5 - 596.5]
      ├─ [596.5 - 598.5]
      ├─ [598.5 - 600.5]
      ├─ [600.5 - 602.5]
      ├─ [602.5 - 604.5]
      ├─ [604.5 - 606.5]
      ├─ [606.5 - 608.5]
      ├─ [608.5 - 610.5]
      ├─ [610.5 - 612.5]
      ├─ [612.5 - 614.5]
      ├─ [614.5 - 616.5]
      ├─ [616.5 - 618.5]
      ├─ [618.5 - 620.5]
      ├─ [620.5 - 622.5]
      ├─ [622.5 - 624.5]
      ├─ [624.5 - 626.5]
      ├─ [626.5 - 628.5]
      ├─ [628.5 - 630.5]
      ├─ [630.5 - 632.5]
      ├─ [632.5 - 634.5]
      ├─ [634.5 - 636.5]
      ├─ [636.5 - 638.5]
      ├─ [638.5 - 640.5]
      ├─ [640.5 - 642.5]
      ├─ [642.5 - 644.5]
      ├─ [644.5 - 646.5]
      ├─ [646.5 - 648.5]
      ├─ [648.5 - 650.5]
      ├─ [650.5 - 652.5]
      ├─ [652.5 - 654.5]
      ├─ [654.5 - 656.5]
      ├─ [656.5 - 658.5]
      ├─ [658.5 - 660.6]
      ├─ [660.6 - 662.6]
      ├─ [662.6 - 664.6]
      ├─ [664.6 - 666.6]
      ├─ [666.6 - 668.6]
      ├─ [668.6 - 670.6]
      ├─ [670.6 - 672.6]
      ├─ [672.6 - 674.6]
      ├─ [674.6 - 676.6]
      ├─ [676.6 - 678.6]
      ├─ [678.6 - 680.6]
      ├─ [680.6 - 682.6]
      ├─ [682.6 - 684.6]
      ├─ [684.6 - 686.6]
      ├─ [686.6 - 688.6]
      ├─ [688.6 - 690.6]
      ├─ [690.6 - 692.6]
      ├─ [692.6 - 694.6]
      ├─ [694.6 - 696.6]
      ├─ [696.6 - 698.6]
      ├─ [698.6 - 700.6]
      ├─ [700.6 - 702.6]
      ├─ [702.6 - 704.6]
      ├─ [704.6 - 706.6]
      ├─ [706.6 - 708.6]
      ├─ [708.6 - 710.6]
      ├─ [710.6 - 712.6]
      ├─ [712.6 - 714.6]
      ├─ [714.6 - 716.6]
      ├─ [716.6 - 718.6]
      ├─ [718.6 - 720.6]
      ├─ [720.6 - 722.6]
      ├─ [722.6 - 724.6]
      ├─ [724.6 - 726.6]
      ├─ [726.6 - 728.6]
      ├─ [728.6 - 730.6]
      ├─ [730.6 - 732.6]
      ├─ [732.6 - 734.6]
      ├─ [734.6 - 736.6]
      ├─ [736.6 - 738.6]
      ├─ [738.6 - 740.6]
      ├─ [740.6 - 742.6]
      ├─ [742.6 - 744.6]
      ├─ [744.6 - 746.6]
      ├─ [746.6 - 748.6]
      ├─ [748.6 - 750.6]
      ├─ [750.6 - 752.6]
      ├─ [752.6 - 754.6]
      ├─ [754.6 - 756.6]
      ├─ [756.6 - 758.6]
      ├─ [758.6 - 760.6]
      ├─ [760.6 - 762.6]
      ├─ [762.6 - 764.6]
      ├─ [764.6 - 766.6]
      ├─ [766.6 - 768.6]
      ├─ [768.6 - 770.6]
      ├─ [770.6 - 772.6]
      ├─ [772.6 - 774.6]
      ├─ [774.6 - 776.6]
      ├─ [776.6 - 778.6]
      ├─ [778.6 - 780.6]
      ├─ [780.6 - 782.6]
      ├─ [782.6 - 784.6]
      ├─ [784.6 - 786.6]
      ├─ [786.6 - 788.6]
      ├─ [788.6 - 790.6]
      ├─ [790.6 - 792.6]
      ├─ [792.6 - 794.6]
      ├─ [794.6 - 796.6]
      ├─ [796.6 - 798.6]
      ├─ [798.6 - 800.6]
      ├─ [800.6 - 802.6]
      ├─ [802.6 - 804.6]
      ├─ [804.6 - 806.6]
      ├─ [806.6 - 808.6]
      ├─ [808.6 - 810.6]
      ├─ [810.6 - 812.6]
      ├─ [812.6 - 814.6]
      ├─ [814.6 - 816.6]
      ├─ [816.6 - 818.6]
      ├─ [818.6 - 820.6]
      ├─ [820.6 - 822.6]
      ├─ [822.6 - 824.6]
      ├─ [824.6 - 826.6]
      ├─ [826.6 - 828.6]
      ├─ [828.6 - 830.6]
      ├─ [830.6 - 832.6]
      ├─ [832.6 - 834.6]
      ├─ [834.6 - 836.6]
      ├─ [836.6 - 838.6]
      ├─ [838.6 - 840.6]
      ├─ [840.6 - 842.6]
      ├─ [842.6 - 844.6]
      ├─ [844.6 - 846.6]
      ├─ [846.6 - 848.6]
      ├─ [848.6 - 850.6]
      ├─ [850.6 - 852.6]
      ├─ [852.6 - 854.6]
      ├─ [854.6 - 856.6]
      ├─ [856.6 - 858.6]
      ├─ [858.6 - 860.6]
      ├─ [860.6 - 862.6]
      ├─ [862.6 - 864.6]
      ├─ [864.6 - 866.6]
      ├─ [866.6 - 868.6]
      ├─ [868.6 - 870.6]
      ├─ [870.6 - 872.6]
      ├─ [872.6 - 874.6]
      ├─ [874.6 - 876.6]
      ├─ [876.6 - 878.6]
      ├─ [878.6 - 880.7]
      ├─ [880.7 - 882.7]
      ├─ [882.7 - 884.7]
      ├─ [884.7 - 886.7]
      ├─ [886.7 - 888.7]
      ├─ [888.7 - 890.7]
      ├─ [890.7 - 892.7]
      ├─ [892.7 - 894.7]
      ├─ [894.7 - 896.7]
      ├─ [896.7 - 898.7]
      ├─ [898.7 - 900.7]
      ├─ [900.7 - 902.7]
      ├─ [902.7 - 904.7]
      ├─ [904.7 - 906.7]
      ├─ [906.7 - 908.7]
      ├─ [908.7 - 910.7]
      ├─ [910.7 - 912.7]
      ├─ [912.7 - 914.7]
      ├─ [914.7 - 916.7]
      ├─ [916.7 - 918.7]
      ├─ [918.7 - 920.7]
      ├─ [920.7 - 922.7]
      ├─ [922.7 - 924.7]
      ├─ [924.7 - 926.7]
      ├─ [926.7 - 928.7]
      ├─ [928.7 - 930.7]
      ├─ [930.7 - 932.7]
      ├─ [932.7 - 934.7]
      ├─ [934.7 - 936.7]
      ├─ [936.7 - 938.7]
      ├─ [938.7 - 940.7]
      ├─ [940.7 - 942.7]
      ├─ [942.7 - 944.7]
      ├─ [944.7 - 946.7]
      ├─ [946.7 - 948.7]
      ├─ [948.7 - 950.7]
      ├─ [950.7 - 952.7]
      ├─ [952.7 - 954.7]
      ├─ [954.7 - 956.7]
      ├─ [956.7 - 958.7]
      ├─ [958.7 - 960.7]
      ├─ [960.7 - 962.7]
      ├─ [962.7 - 964.7]
      ├─ [964.7 - 966.7]
      ├─ [966.7 - 968.7]
      ├─ [968.7 - 970.7]
      ├─ [970.7 - 972.7]
      ├─ [972.7 - 974.7]
      ├─ [974.7 - 976.7]
      ├─ [976.7 - 978.7]
      └─ [978.7 - 980.7]

Run: LFQ_Astral_DIA_15min_50ng_Condition_B_REP2.raw
   ├─ Vendor: Thermo
   ├─ File: LFQ_Astral_DIA_15min_50ng_Condition_B_REP2
   ├─ Path: "\\tol-brandir\Masse\Public datasets\ProteoBench\Module_quant_DIA_Astral\LFQ_Astral_DIA_15min_50ng_Condition_B_REP2.raw"
   ├─ Condition: Not Defined
   ├─ Replicate: 5
   ├─ HTRMS Version: 21.0.260602.94842
   ├─ Protein Databases Used
   │  └─ ProteoBenchFASTA_MixedSpecies_HYE
   │     ├─ Protein Entries: 31 889
   │     ├─ Original File: ProteoBenchFASTA_MixedSpecies_HYE.fasta
   │     ├─ Date Created: 24-February-2026 09:47:25 UTC +01:00 
   │     ├─ Date Modified: 31-July-2026 15:15:38 UTC +02:00 
   │     └─ Parsing Rule: UniProt FASTA
   └─ MS2 Method
      ├─ [380.4 - 382.4]
      ├─ [382.4 - 384.4]
      ├─ [384.4 - 386.4]
      ├─ [386.4 - 388.4]
      ├─ [388.4 - 390.4]
      ├─ [390.4 - 392.4]
      ├─ [392.4 - 394.4]
      ├─ [394.4 - 396.4]
      ├─ [396.4 - 398.4]
      ├─ [398.4 - 400.4]
      ├─ [400.4 - 402.4]
      ├─ [402.4 - 404.4]
      ├─ [404.4 - 406.4]
      ├─ [406.4 - 408.4]
      ├─ [408.4 - 410.4]
      ├─ [410.4 - 412.4]
      ├─ [412.4 - 414.4]
      ├─ [414.4 - 416.4]
      ├─ [416.4 - 418.4]
      ├─ [418.4 - 420.4]
      ├─ [420.4 - 422.4]
      ├─ [422.4 - 424.4]
      ├─ [424.4 - 426.4]
      ├─ [426.4 - 428.4]
      ├─ [428.4 - 430.4]
      ├─ [430.4 - 432.4]
      ├─ [432.4 - 434.4]
      ├─ [434.4 - 436.4]
      ├─ [436.4 - 438.4]
      ├─ [438.4 - 440.5]
      ├─ [440.5 - 442.5]
      ├─ [442.5 - 444.5]
      ├─ [444.5 - 446.5]
      ├─ [446.5 - 448.5]
      ├─ [448.5 - 450.5]
      ├─ [450.5 - 452.5]
      ├─ [452.5 - 454.5]
      ├─ [454.5 - 456.5]
      ├─ [456.5 - 458.5]
      ├─ [458.5 - 460.5]
      ├─ [460.5 - 462.5]
      ├─ [462.5 - 464.5]
      ├─ [464.5 - 466.5]
      ├─ [466.5 - 468.5]
      ├─ [468.5 - 470.5]
      ├─ [470.5 - 472.5]
      ├─ [472.5 - 474.5]
      ├─ [474.5 - 476.5]
      ├─ [476.5 - 478.5]
      ├─ [478.5 - 480.5]
      ├─ [480.5 - 482.5]
      ├─ [482.5 - 484.5]
      ├─ [484.5 - 486.5]
      ├─ [486.5 - 488.5]
      ├─ [488.5 - 490.5]
      ├─ [490.5 - 492.5]
      ├─ [492.5 - 494.5]
      ├─ [494.5 - 496.5]
      ├─ [496.5 - 498.5]
      ├─ [498.5 - 500.5]
      ├─ [500.5 - 502.5]
      ├─ [502.5 - 504.5]
      ├─ [504.5 - 506.5]
      ├─ [506.5 - 508.5]
      ├─ [508.5 - 510.5]
      ├─ [510.5 - 512.5]
      ├─ [512.5 - 514.5]
      ├─ [514.5 - 516.5]
      ├─ [516.5 - 518.5]
      ├─ [518.5 - 520.5]
      ├─ [520.5 - 522.5]
      ├─ [522.5 - 524.5]
      ├─ [524.5 - 526.5]
      ├─ [526.5 - 528.5]
      ├─ [528.5 - 530.5]
      ├─ [530.5 - 532.5]
      ├─ [532.5 - 534.5]
      ├─ [534.5 - 536.5]
      ├─ [536.5 - 538.5]
      ├─ [538.5 - 540.5]
      ├─ [540.5 - 542.5]
      ├─ [542.5 - 544.5]
      ├─ [544.5 - 546.5]
      ├─ [546.5 - 548.5]
      ├─ [548.5 - 550.5]
      ├─ [550.5 - 552.5]
      ├─ [552.5 - 554.5]
      ├─ [554.5 - 556.5]
      ├─ [556.5 - 558.5]
      ├─ [558.5 - 560.5]
      ├─ [560.5 - 562.5]
      ├─ [562.5 - 564.5]
      ├─ [564.5 - 566.5]
      ├─ [566.5 - 568.5]
      ├─ [568.5 - 570.5]
      ├─ [570.5 - 572.5]
      ├─ [572.5 - 574.5]
      ├─ [574.5 - 576.5]
      ├─ [576.5 - 578.5]
      ├─ [578.5 - 580.5]
      ├─ [580.5 - 582.5]
      ├─ [582.5 - 584.5]
      ├─ [584.5 - 586.5]
      ├─ [586.5 - 588.5]
      ├─ [588.5 - 590.5]
      ├─ [590.5 - 592.5]
      ├─ [592.5 - 594.5]
      ├─ [594.5 - 596.5]
      ├─ [596.5 - 598.5]
      ├─ [598.5 - 600.5]
      ├─ [600.5 - 602.5]
      ├─ [602.5 - 604.5]
      ├─ [604.5 - 606.5]
      ├─ [606.5 - 608.5]
      ├─ [608.5 - 610.5]
      ├─ [610.5 - 612.5]
      ├─ [612.5 - 614.5]
      ├─ [614.5 - 616.5]
      ├─ [616.5 - 618.5]
      ├─ [618.5 - 620.5]
      ├─ [620.5 - 622.5]
      ├─ [622.5 - 624.5]
      ├─ [624.5 - 626.5]
      ├─ [626.5 - 628.5]
      ├─ [628.5 - 630.5]
      ├─ [630.5 - 632.5]
      ├─ [632.5 - 634.5]
      ├─ [634.5 - 636.5]
      ├─ [636.5 - 638.5]
      ├─ [638.5 - 640.5]
      ├─ [640.5 - 642.5]
      ├─ [642.5 - 644.5]
      ├─ [644.5 - 646.5]
      ├─ [646.5 - 648.5]
      ├─ [648.5 - 650.5]
      ├─ [650.5 - 652.5]
      ├─ [652.5 - 654.5]
      ├─ [654.5 - 656.5]
      ├─ [656.5 - 658.5]
      ├─ [658.5 - 660.6]
      ├─ [660.6 - 662.6]
      ├─ [662.6 - 664.6]
      ├─ [664.6 - 666.6]
      ├─ [666.6 - 668.6]
      ├─ [668.6 - 670.6]
      ├─ [670.6 - 672.6]
      ├─ [672.6 - 674.6]
      ├─ [674.6 - 676.6]
      ├─ [676.6 - 678.6]
      ├─ [678.6 - 680.6]
      ├─ [680.6 - 682.6]
      ├─ [682.6 - 684.6]
      ├─ [684.6 - 686.6]
      ├─ [686.6 - 688.6]
      ├─ [688.6 - 690.6]
      ├─ [690.6 - 692.6]
      ├─ [692.6 - 694.6]
      ├─ [694.6 - 696.6]
      ├─ [696.6 - 698.6]
      ├─ [698.6 - 700.6]
      ├─ [700.6 - 702.6]
      ├─ [702.6 - 704.6]
      ├─ [704.6 - 706.6]
      ├─ [706.6 - 708.6]
      ├─ [708.6 - 710.6]
      ├─ [710.6 - 712.6]
      ├─ [712.6 - 714.6]
      ├─ [714.6 - 716.6]
      ├─ [716.6 - 718.6]
      ├─ [718.6 - 720.6]
      ├─ [720.6 - 722.6]
      ├─ [722.6 - 724.6]
      ├─ [724.6 - 726.6]
      ├─ [726.6 - 728.6]
      ├─ [728.6 - 730.6]
      ├─ [730.6 - 732.6]
      ├─ [732.6 - 734.6]
      ├─ [734.6 - 736.6]
      ├─ [736.6 - 738.6]
      ├─ [738.6 - 740.6]
      ├─ [740.6 - 742.6]
      ├─ [742.6 - 744.6]
      ├─ [744.6 - 746.6]
      ├─ [746.6 - 748.6]
      ├─ [748.6 - 750.6]
      ├─ [750.6 - 752.6]
      ├─ [752.6 - 754.6]
      ├─ [754.6 - 756.6]
      ├─ [756.6 - 758.6]
      ├─ [758.6 - 760.6]
      ├─ [760.6 - 762.6]
      ├─ [762.6 - 764.6]
      ├─ [764.6 - 766.6]
      ├─ [766.6 - 768.6]
      ├─ [768.6 - 770.6]
      ├─ [770.6 - 772.6]
      ├─ [772.6 - 774.6]
      ├─ [774.6 - 776.6]
      ├─ [776.6 - 778.6]
      ├─ [778.6 - 780.6]
      ├─ [780.6 - 782.6]
      ├─ [782.6 - 784.6]
      ├─ [784.6 - 786.6]
      ├─ [786.6 - 788.6]
      ├─ [788.6 - 790.6]
      ├─ [790.6 - 792.6]
      ├─ [792.6 - 794.6]
      ├─ [794.6 - 796.6]
      ├─ [796.6 - 798.6]
      ├─ [798.6 - 800.6]
      ├─ [800.6 - 802.6]
      ├─ [802.6 - 804.6]
      ├─ [804.6 - 806.6]
      ├─ [806.6 - 808.6]
      ├─ [808.6 - 810.6]
      ├─ [810.6 - 812.6]
      ├─ [812.6 - 814.6]
      ├─ [814.6 - 816.6]
      ├─ [816.6 - 818.6]
      ├─ [818.6 - 820.6]
      ├─ [820.6 - 822.6]
      ├─ [822.6 - 824.6]
      ├─ [824.6 - 826.6]
      ├─ [826.6 - 828.6]
      ├─ [828.6 - 830.6]
      ├─ [830.6 - 832.6]
      ├─ [832.6 - 834.6]
      ├─ [834.6 - 836.6]
      ├─ [836.6 - 838.6]
      ├─ [838.6 - 840.6]
      ├─ [840.6 - 842.6]
      ├─ [842.6 - 844.6]
      ├─ [844.6 - 846.6]
      ├─ [846.6 - 848.6]
      ├─ [848.6 - 850.6]
      ├─ [850.6 - 852.6]
      ├─ [852.6 - 854.6]
      ├─ [854.6 - 856.6]
      ├─ [856.6 - 858.6]
      ├─ [858.6 - 860.6]
      ├─ [860.6 - 862.6]
      ├─ [862.6 - 864.6]
      ├─ [864.6 - 866.6]
      ├─ [866.6 - 868.6]
      ├─ [868.6 - 870.6]
      ├─ [870.6 - 872.6]
      ├─ [872.6 - 874.6]
      ├─ [874.6 - 876.6]
      ├─ [876.6 - 878.6]
      ├─ [878.6 - 880.7]
      ├─ [880.7 - 882.7]
      ├─ [882.7 - 884.7]
      ├─ [884.7 - 886.7]
      ├─ [886.7 - 888.7]
      ├─ [888.7 - 890.7]
      ├─ [890.7 - 892.7]
      ├─ [892.7 - 894.7]
      ├─ [894.7 - 896.7]
      ├─ [896.7 - 898.7]
      ├─ [898.7 - 900.7]
      ├─ [900.7 - 902.7]
      ├─ [902.7 - 904.7]
      ├─ [904.7 - 906.7]
      ├─ [906.7 - 908.7]
      ├─ [908.7 - 910.7]
      ├─ [910.7 - 912.7]
      ├─ [912.7 - 914.7]
      ├─ [914.7 - 916.7]
      ├─ [916.7 - 918.7]
      ├─ [918.7 - 920.7]
      ├─ [920.7 - 922.7]
      ├─ [922.7 - 924.7]
      ├─ [924.7 - 926.7]
      ├─ [926.7 - 928.7]
      ├─ [928.7 - 930.7]
      ├─ [930.7 - 932.7]
      ├─ [932.7 - 934.7]
      ├─ [934.7 - 936.7]
      ├─ [936.7 - 938.7]
      ├─ [938.7 - 940.7]
      ├─ [940.7 - 942.7]
      ├─ [942.7 - 944.7]
      ├─ [944.7 - 946.7]
      ├─ [946.7 - 948.7]
      ├─ [948.7 - 950.7]
      ├─ [950.7 - 952.7]
      ├─ [952.7 - 954.7]
      ├─ [954.7 - 956.7]
      ├─ [956.7 - 958.7]
      ├─ [958.7 - 960.7]
      ├─ [960.7 - 962.7]
      ├─ [962.7 - 964.7]
      ├─ [964.7 - 966.7]
      ├─ [966.7 - 968.7]
      ├─ [968.7 - 970.7]
      ├─ [970.7 - 972.7]
      ├─ [972.7 - 974.7]
      ├─ [974.7 - 976.7]
      ├─ [976.7 - 978.7]
      └─ [978.7 - 980.7]

Run: LFQ_Astral_DIA_15min_50ng_Condition_B_REP3.raw
   ├─ Vendor: Thermo
   ├─ File: LFQ_Astral_DIA_15min_50ng_Condition_B_REP3
   ├─ Path: "\\tol-brandir\Masse\Public datasets\ProteoBench\Module_quant_DIA_Astral\LFQ_Astral_DIA_15min_50ng_Condition_B_REP3.raw"
   ├─ Condition: Not Defined
   ├─ Replicate: 6
   ├─ HTRMS Version: 21.0.260602.94842
   ├─ Protein Databases Used
   │  └─ ProteoBenchFASTA_MixedSpecies_HYE
   │     ├─ Protein Entries: 31 889
   │     ├─ Original File: ProteoBenchFASTA_MixedSpecies_HYE.fasta
   │     ├─ Date Created: 24-February-2026 09:47:25 UTC +01:00 
   │     ├─ Date Modified: 31-July-2026 15:15:38 UTC +02:00 
   │     └─ Parsing Rule: UniProt FASTA
   └─ MS2 Method
      ├─ [380.4 - 382.4]
      ├─ [382.4 - 384.4]
      ├─ [384.4 - 386.4]
      ├─ [386.4 - 388.4]
      ├─ [388.4 - 390.4]
      ├─ [390.4 - 392.4]
      ├─ [392.4 - 394.4]
      ├─ [394.4 - 396.4]
      ├─ [396.4 - 398.4]
      ├─ [398.4 - 400.4]
      ├─ [400.4 - 402.4]
      ├─ [402.4 - 404.4]
      ├─ [404.4 - 406.4]
      ├─ [406.4 - 408.4]
      ├─ [408.4 - 410.4]
      ├─ [410.4 - 412.4]
      ├─ [412.4 - 414.4]
      ├─ [414.4 - 416.4]
      ├─ [416.4 - 418.4]
      ├─ [418.4 - 420.4]
      ├─ [420.4 - 422.4]
      ├─ [422.4 - 424.4]
      ├─ [424.4 - 426.4]
      ├─ [426.4 - 428.4]
      ├─ [428.4 - 430.4]
      ├─ [430.4 - 432.4]
      ├─ [432.4 - 434.4]
      ├─ [434.4 - 436.4]
      ├─ [436.4 - 438.4]
      ├─ [438.4 - 440.5]
      ├─ [440.5 - 442.5]
      ├─ [442.5 - 444.5]
      ├─ [444.5 - 446.5]
      ├─ [446.5 - 448.5]
      ├─ [448.5 - 450.5]
      ├─ [450.5 - 452.5]
      ├─ [452.5 - 454.5]
      ├─ [454.5 - 456.5]
      ├─ [456.5 - 458.5]
      ├─ [458.5 - 460.5]
      ├─ [460.5 - 462.5]
      ├─ [462.5 - 464.5]
      ├─ [464.5 - 466.5]
      ├─ [466.5 - 468.5]
      ├─ [468.5 - 470.5]
      ├─ [470.5 - 472.5]
      ├─ [472.5 - 474.5]
      ├─ [474.5 - 476.5]
      ├─ [476.5 - 478.5]
      ├─ [478.5 - 480.5]
      ├─ [480.5 - 482.5]
      ├─ [482.5 - 484.5]
      ├─ [484.5 - 486.5]
      ├─ [486.5 - 488.5]
      ├─ [488.5 - 490.5]
      ├─ [490.5 - 492.5]
      ├─ [492.5 - 494.5]
      ├─ [494.5 - 496.5]
      ├─ [496.5 - 498.5]
      ├─ [498.5 - 500.5]
      ├─ [500.5 - 502.5]
      ├─ [502.5 - 504.5]
      ├─ [504.5 - 506.5]
      ├─ [506.5 - 508.5]
      ├─ [508.5 - 510.5]
      ├─ [510.5 - 512.5]
      ├─ [512.5 - 514.5]
      ├─ [514.5 - 516.5]
      ├─ [516.5 - 518.5]
      ├─ [518.5 - 520.5]
      ├─ [520.5 - 522.5]
      ├─ [522.5 - 524.5]
      ├─ [524.5 - 526.5]
      ├─ [526.5 - 528.5]
      ├─ [528.5 - 530.5]
      ├─ [530.5 - 532.5]
      ├─ [532.5 - 534.5]
      ├─ [534.5 - 536.5]
      ├─ [536.5 - 538.5]
      ├─ [538.5 - 540.5]
      ├─ [540.5 - 542.5]
      ├─ [542.5 - 544.5]
      ├─ [544.5 - 546.5]
      ├─ [546.5 - 548.5]
      ├─ [548.5 - 550.5]
      ├─ [550.5 - 552.5]
      ├─ [552.5 - 554.5]
      ├─ [554.5 - 556.5]
      ├─ [556.5 - 558.5]
      ├─ [558.5 - 560.5]
      ├─ [560.5 - 562.5]
      ├─ [562.5 - 564.5]
      ├─ [564.5 - 566.5]
      ├─ [566.5 - 568.5]
      ├─ [568.5 - 570.5]
      ├─ [570.5 - 572.5]
      ├─ [572.5 - 574.5]
      ├─ [574.5 - 576.5]
      ├─ [576.5 - 578.5]
      ├─ [578.5 - 580.5]
      ├─ [580.5 - 582.5]
      ├─ [582.5 - 584.5]
      ├─ [584.5 - 586.5]
      ├─ [586.5 - 588.5]
      ├─ [588.5 - 590.5]
      ├─ [590.5 - 592.5]
      ├─ [592.5 - 594.5]
      ├─ [594.5 - 596.5]
      ├─ [596.5 - 598.5]
      ├─ [598.5 - 600.5]
      ├─ [600.5 - 602.5]
      ├─ [602.5 - 604.5]
      ├─ [604.5 - 606.5]
      ├─ [606.5 - 608.5]
      ├─ [608.5 - 610.5]
      ├─ [610.5 - 612.5]
      ├─ [612.5 - 614.5]
      ├─ [614.5 - 616.5]
      ├─ [616.5 - 618.5]
      ├─ [618.5 - 620.5]
      ├─ [620.5 - 622.5]
      ├─ [622.5 - 624.5]
      ├─ [624.5 - 626.5]
      ├─ [626.5 - 628.5]
      ├─ [628.5 - 630.5]
      ├─ [630.5 - 632.5]
      ├─ [632.5 - 634.5]
      ├─ [634.5 - 636.5]
      ├─ [636.5 - 638.5]
      ├─ [638.5 - 640.5]
      ├─ [640.5 - 642.5]
      ├─ [642.5 - 644.5]
      ├─ [644.5 - 646.5]
      ├─ [646.5 - 648.5]
      ├─ [648.5 - 650.5]
      ├─ [650.5 - 652.5]
      ├─ [652.5 - 654.5]
      ├─ [654.5 - 656.5]
      ├─ [656.5 - 658.5]
      ├─ [658.5 - 660.6]
      ├─ [660.6 - 662.6]
      ├─ [662.6 - 664.6]
      ├─ [664.6 - 666.6]
      ├─ [666.6 - 668.6]
      ├─ [668.6 - 670.6]
      ├─ [670.6 - 672.6]
      ├─ [672.6 - 674.6]
      ├─ [674.6 - 676.6]
      ├─ [676.6 - 678.6]
      ├─ [678.6 - 680.6]
      ├─ [680.6 - 682.6]
      ├─ [682.6 - 684.6]
      ├─ [684.6 - 686.6]
      ├─ [686.6 - 688.6]
      ├─ [688.6 - 690.6]
      ├─ [690.6 - 692.6]
      ├─ [692.6 - 694.6]
      ├─ [694.6 - 696.6]
      ├─ [696.6 - 698.6]
      ├─ [698.6 - 700.6]
      ├─ [700.6 - 702.6]
      ├─ [702.6 - 704.6]
      ├─ [704.6 - 706.6]
      ├─ [706.6 - 708.6]
      ├─ [708.6 - 710.6]
      ├─ [710.6 - 712.6]
      ├─ [712.6 - 714.6]
      ├─ [714.6 - 716.6]
      ├─ [716.6 - 718.6]
      ├─ [718.6 - 720.6]
      ├─ [720.6 - 722.6]
      ├─ [722.6 - 724.6]
      ├─ [724.6 - 726.6]
      ├─ [726.6 - 728.6]
      ├─ [728.6 - 730.6]
      ├─ [730.6 - 732.6]
      ├─ [732.6 - 734.6]
      ├─ [734.6 - 736.6]
      ├─ [736.6 - 738.6]
      ├─ [738.6 - 740.6]
      ├─ [740.6 - 742.6]
      ├─ [742.6 - 744.6]
      ├─ [744.6 - 746.6]
      ├─ [746.6 - 748.6]
      ├─ [748.6 - 750.6]
      ├─ [750.6 - 752.6]
      ├─ [752.6 - 754.6]
      ├─ [754.6 - 756.6]
      ├─ [756.6 - 758.6]
      ├─ [758.6 - 760.6]
      ├─ [760.6 - 762.6]
      ├─ [762.6 - 764.6]
      ├─ [764.6 - 766.6]
      ├─ [766.6 - 768.6]
      ├─ [768.6 - 770.6]
      ├─ [770.6 - 772.6]
      ├─ [772.6 - 774.6]
      ├─ [774.6 - 776.6]
      ├─ [776.6 - 778.6]
      ├─ [778.6 - 780.6]
      ├─ [780.6 - 782.6]
      ├─ [782.6 - 784.6]
      ├─ [784.6 - 786.6]
      ├─ [786.6 - 788.6]
      ├─ [788.6 - 790.6]
      ├─ [790.6 - 792.6]
      ├─ [792.6 - 794.6]
      ├─ [794.6 - 796.6]
      ├─ [796.6 - 798.6]
      ├─ [798.6 - 800.6]
      ├─ [800.6 - 802.6]
      ├─ [802.6 - 804.6]
      ├─ [804.6 - 806.6]
      ├─ [806.6 - 808.6]
      ├─ [808.6 - 810.6]
      ├─ [810.6 - 812.6]
      ├─ [812.6 - 814.6]
      ├─ [814.6 - 816.6]
      ├─ [816.6 - 818.6]
      ├─ [818.6 - 820.6]
      ├─ [820.6 - 822.6]
      ├─ [822.6 - 824.6]
      ├─ [824.6 - 826.6]
      ├─ [826.6 - 828.6]
      ├─ [828.6 - 830.6]
      ├─ [830.6 - 832.6]
      ├─ [832.6 - 834.6]
      ├─ [834.6 - 836.6]
      ├─ [836.6 - 838.6]
      ├─ [838.6 - 840.6]
      ├─ [840.6 - 842.6]
      ├─ [842.6 - 844.6]
      ├─ [844.6 - 846.6]
      ├─ [846.6 - 848.6]
      ├─ [848.6 - 850.6]
      ├─ [850.6 - 852.6]
      ├─ [852.6 - 854.6]
      ├─ [854.6 - 856.6]
      ├─ [856.6 - 858.6]
      ├─ [858.6 - 860.6]
      ├─ [860.6 - 862.6]
      ├─ [862.6 - 864.6]
      ├─ [864.6 - 866.6]
      ├─ [866.6 - 868.6]
      ├─ [868.6 - 870.6]
      ├─ [870.6 - 872.6]
      ├─ [872.6 - 874.6]
      ├─ [874.6 - 876.6]
      ├─ [876.6 - 878.6]
      ├─ [878.6 - 880.7]
      ├─ [880.7 - 882.7]
      ├─ [882.7 - 884.7]
      ├─ [884.7 - 886.7]
      ├─ [886.7 - 888.7]
      ├─ [888.7 - 890.7]
      ├─ [890.7 - 892.7]
      ├─ [892.7 - 894.7]
      ├─ [894.7 - 896.7]
      ├─ [896.7 - 898.7]
      ├─ [898.7 - 900.7]
      ├─ [900.7 - 902.7]
      ├─ [902.7 - 904.7]
      ├─ [904.7 - 906.7]
      ├─ [906.7 - 908.7]
      ├─ [908.7 - 910.7]
      ├─ [910.7 - 912.7]
      ├─ [912.7 - 914.7]
      ├─ [914.7 - 916.7]
      ├─ [916.7 - 918.7]
      ├─ [918.7 - 920.7]
      ├─ [920.7 - 922.7]
      ├─ [922.7 - 924.7]
      ├─ [924.7 - 926.7]
      ├─ [926.7 - 928.7]
      ├─ [928.7 - 930.7]
      ├─ [930.7 - 932.7]
      ├─ [932.7 - 934.7]
      ├─ [934.7 - 936.7]
      ├─ [936.7 - 938.7]
      ├─ [938.7 - 940.7]
      ├─ [940.7 - 942.7]
      ├─ [942.7 - 944.7]
      ├─ [944.7 - 946.7]
      ├─ [946.7 - 948.7]
      ├─ [948.7 - 950.7]
      ├─ [950.7 - 952.7]
      ├─ [952.7 - 954.7]
      ├─ [954.7 - 956.7]
      ├─ [956.7 - 958.7]
      ├─ [958.7 - 960.7]
      ├─ [960.7 - 962.7]
      ├─ [962.7 - 964.7]
      ├─ [964.7 - 966.7]
      ├─ [966.7 - 968.7]
      ├─ [968.7 - 970.7]
      ├─ [970.7 - 972.7]
      ├─ [972.7 - 974.7]
      ├─ [974.7 - 976.7]
      ├─ [976.7 - 978.7]
      └─ [978.7 - 980.7]

[END-SETUP]

[BEGIN-LOG]
INFO:    [31/07/2026 16:30:05] -> Initializing Pipeline...
INFO:    [31/07/2026 16:30:05] -> Loading...
INFO:    [31/07/2026 16:30:05] -> Loading SNE File: D:\Utilisateurs\locard\SpectronautFiles\20260831_ProteoBench_DIAQuantAstralModule\20260731_100134_20260831_Quant_Astral_DIA_forRobbe\20260731_100134_20260831_Quant_Astral_DIA_forRobbe.sne
INFO:    [31/07/2026 10:01:39] -> Initializing Pipeline...
INFO:    [31/07/2026 10:01:39] -> Preprocessing Run #1 of 6...
INFO:    [31/07/2026 10:01:40] -> Searching DIA with Pulsar...
INFO:    [31/07/2026 10:01:50] -> Initialize Pipeline...
INFO:    [31/07/2026 10:01:50] -> Initialize Pipeline
INFO:    [31/07/2026 10:02:30] -> Initialize Pipeline...
INFO:    [31/07/2026 10:02:30] -> Creating Experiment Environment
INFO:    [31/07/2026 10:02:30] -> SuperRun 1/6: Initializing (LFQ_Astral_DIA_15min_50ng_Condition_A_REP1.raw)
INFO:    [31/07/2026 10:02:30] -> Run 1/6: Organizing Data from Run
INFO:    [31/07/2026 10:02:31] -> Run 1/6: Creating Calibration Search Space...
INFO:    [31/07/2026 10:02:34] -> Collecting Search Space Parameters...
INFO:    [31/07/2026 10:02:51] -> Creating the Search Space...
INFO:    [31/07/2026 10:03:12] -> Run 1/6: Creating Search Space...
INFO:    [31/07/2026 10:03:12] -> Collecting Search Space Parameters...
INFO:    [31/07/2026 10:03:41] -> Creating the Search Space...
INFO:    [31/07/2026 10:03:57] -> Run 1/6: MS2 Index Generation
INFO:    [31/07/2026 10:08:51] -> MS2 Index Generation Time: 4.89m
INFO:    [31/07/2026 10:08:51] -> Run 1/6: Method-Specific Pre-Processing...
INFO:    [31/07/2026 10:09:02] -> Initializing...
INFO:    [31/07/2026 10:12:50] -> Finalizing Scan Map...
INFO:    [31/07/2026 10:18:22] -> Processed in 9.3m
INFO:    [31/07/2026 10:18:32] -> Done
INFO:    [31/07/2026 10:19:41] -> Run 1/6: Process Raw File...
INFO:    [31/07/2026 10:19:54] -> Run 1/6: Preparing Partitions...
INFO:    [31/07/2026 10:19:54] -> Part 1/1 - Run 1/6: Preparing Calibration Searches...
INFO:    [31/07/2026 10:19:56] -> Part 1/1 - Run 1/6: First Pass Calibration Search...
INFO:    [31/07/2026 10:20:43] -> Part 1/1 - Run 1/6: Calibration Search...
INFO:    [31/07/2026 10:21:12] -> The number of PSMs identified during calibration with FDR <= 0.01 is 5839 [29.1s]
INFO:    [31/07/2026 10:25:57] -> Part 1/1 - Run 1/6: Preparing Main Search...
INFO:    [31/07/2026 10:25:58] -> Part 1/1 - Run 1/6: Main Search...
INFO:    [31/07/2026 10:28:42] -> Extracting 425766 MS1 XICs
INFO:    [31/07/2026 10:34:31] -> Run 1/6: Score Post-Processing
INFO:    [31/07/2026 10:36:53] -> Run 1/6: Cleaning Up Run...
INFO:    [31/07/2026 10:37:00] -> SuperRun 2/6: Initializing (LFQ_Astral_DIA_15min_50ng_Condition_A_REP2.raw)
INFO:    [31/07/2026 10:37:00] -> Run 2/6: Organizing Data from Run
INFO:    [31/07/2026 10:37:00] -> Run 2/6: Creating Calibration Search Space...
INFO:    [31/07/2026 10:37:00] -> Run 2/6: Creating Search Space...
INFO:    [31/07/2026 10:37:00] -> Run 2/6: MS2 Index Generation
INFO:    [31/07/2026 10:37:01] -> MS2 Index Generation Time: 1.39s
INFO:    [31/07/2026 10:37:01] -> Run 2/6: Method-Specific Pre-Processing...
INFO:    [31/07/2026 10:37:03] -> Initializing...
INFO:    [31/07/2026 10:37:52] -> Finalizing Scan Map...
INFO:    [31/07/2026 10:39:08] -> Processed in 2.1m
INFO:    [31/07/2026 10:39:14] -> Done
INFO:    [31/07/2026 10:39:21] -> Run 2/6: Process Raw File...
INFO:    [31/07/2026 10:39:26] -> Run 2/6: Preparing Partitions...
INFO:    [31/07/2026 10:39:26] -> Part 1/1 - Run 2/6: Preparing Calibration Searches...
INFO:    [31/07/2026 10:39:28] -> Part 1/1 - Run 2/6: First Pass Calibration Search...
INFO:    [31/07/2026 10:39:41] -> Part 1/1 - Run 2/6: Calibration Search...
INFO:    [31/07/2026 10:39:46] -> The number of PSMs identified during calibration with FDR <= 0.01 is 6163 [5.5s]
INFO:    [31/07/2026 10:40:17] -> Part 1/1 - Run 2/6: Preparing Main Search...
INFO:    [31/07/2026 10:40:18] -> Part 1/1 - Run 2/6: Main Search...
INFO:    [31/07/2026 10:43:04] -> Extracting 428571 MS1 XICs
INFO:    [31/07/2026 10:57:23] -> Run 2/6: Score Post-Processing
INFO:    [31/07/2026 11:25:48] -> Run 2/6: Cleaning Up Run...
INFO:    [31/07/2026 11:25:55] -> SuperRun 3/6: Initializing (LFQ_Astral_DIA_15min_50ng_Condition_A_REP3.raw)
INFO:    [31/07/2026 11:25:55] -> Run 3/6: Organizing Data from Run
INFO:    [31/07/2026 11:25:55] -> Run 3/6: Creating Calibration Search Space...
INFO:    [31/07/2026 11:25:55] -> Run 3/6: Creating Search Space...
INFO:    [31/07/2026 11:25:55] -> Run 3/6: MS2 Index Generation
INFO:    [31/07/2026 11:25:56] -> MS2 Index Generation Time: 1.37s
INFO:    [31/07/2026 11:25:56] -> Run 3/6: Method-Specific Pre-Processing...
INFO:    [31/07/2026 11:25:59] -> Initializing...
INFO:    [31/07/2026 11:26:53] -> Finalizing Scan Map...
INFO:    [31/07/2026 11:28:53] -> Processed in 2.9m
INFO:    [31/07/2026 11:28:58] -> Done
INFO:    [31/07/2026 11:29:06] -> Run 3/6: Process Raw File...
INFO:    [31/07/2026 11:29:09] -> Run 3/6: Preparing Partitions...
INFO:    [31/07/2026 11:29:10] -> Part 1/1 - Run 3/6: Preparing Calibration Searches...
INFO:    [31/07/2026 11:29:11] -> Part 1/1 - Run 3/6: First Pass Calibration Search...
INFO:    [31/07/2026 11:29:27] -> Part 1/1 - Run 3/6: Calibration Search...
INFO:    [31/07/2026 11:29:37] -> The number of PSMs identified during calibration with FDR <= 0.01 is 5573 [10.5s]
INFO:    [31/07/2026 11:30:07] -> Part 1/1 - Run 3/6: Preparing Main Search...
INFO:    [31/07/2026 11:30:08] -> Part 1/1 - Run 3/6: Main Search...
INFO:    [31/07/2026 11:34:41] -> Extracting 381775 MS1 XICs
INFO:    [31/07/2026 11:40:43] -> Run 3/6: Score Post-Processing
INFO:    [31/07/2026 11:45:13] -> Run 3/6: Cleaning Up Run...
INFO:    [31/07/2026 11:45:19] -> SuperRun 4/6: Initializing (LFQ_Astral_DIA_15min_50ng_Condition_B_REP1.raw)
INFO:    [31/07/2026 11:45:19] -> Run 4/6: Organizing Data from Run
INFO:    [31/07/2026 11:45:19] -> Run 4/6: Creating Calibration Search Space...
INFO:    [31/07/2026 11:45:19] -> Run 4/6: Creating Search Space...
INFO:    [31/07/2026 11:45:19] -> Run 4/6: MS2 Index Generation
INFO:    [31/07/2026 11:45:21] -> MS2 Index Generation Time: 1.5s
INFO:    [31/07/2026 11:45:21] -> Run 4/6: Method-Specific Pre-Processing...
INFO:    [31/07/2026 11:45:25] -> Initializing...
INFO:    [31/07/2026 11:46:34] -> Finalizing Scan Map...
INFO:    [31/07/2026 11:48:47] -> Processed in 3.4m
INFO:    [31/07/2026 11:48:52] -> Done
INFO:    [31/07/2026 11:49:03] -> Run 4/6: Process Raw File...
INFO:    [31/07/2026 11:49:06] -> Run 4/6: Preparing Partitions...
INFO:    [31/07/2026 11:49:06] -> Part 1/1 - Run 4/6: Preparing Calibration Searches...
INFO:    [31/07/2026 11:49:08] -> Part 1/1 - Run 4/6: First Pass Calibration Search...
INFO:    [31/07/2026 11:49:24] -> Part 1/1 - Run 4/6: Calibration Search...
INFO:    [31/07/2026 11:49:36] -> The number of PSMs identified during calibration with FDR <= 0.01 is 5987 [11.1s]
INFO:    [31/07/2026 11:50:55] -> Part 1/1 - Run 4/6: Preparing Main Search...
INFO:    [31/07/2026 11:50:57] -> Part 1/1 - Run 4/6: Main Search...
INFO:    [31/07/2026 11:55:49] -> Extracting 422186 MS1 XICs
INFO:    [31/07/2026 12:00:55] -> Run 4/6: Score Post-Processing
INFO:    [31/07/2026 12:03:03] -> Run 4/6: Cleaning Up Run...
INFO:    [31/07/2026 12:03:10] -> SuperRun 5/6: Initializing (LFQ_Astral_DIA_15min_50ng_Condition_B_REP2.raw)
INFO:    [31/07/2026 12:03:10] -> Run 5/6: Organizing Data from Run
INFO:    [31/07/2026 12:03:10] -> Run 5/6: Creating Calibration Search Space...
INFO:    [31/07/2026 12:03:10] -> Run 5/6: Creating Search Space...
INFO:    [31/07/2026 12:03:10] -> Run 5/6: MS2 Index Generation
INFO:    [31/07/2026 12:03:12] -> MS2 Index Generation Time: 1.42s
INFO:    [31/07/2026 12:03:12] -> Run 5/6: Method-Specific Pre-Processing...
INFO:    [31/07/2026 12:03:20] -> Initializing...
INFO:    [31/07/2026 12:04:10] -> Finalizing Scan Map...
INFO:    [31/07/2026 12:05:31] -> Processed in 2.2m
INFO:    [31/07/2026 12:05:36] -> Done
INFO:    [31/07/2026 12:05:43] -> Run 5/6: Process Raw File...
INFO:    [31/07/2026 12:05:49] -> Run 5/6: Preparing Partitions...
INFO:    [31/07/2026 12:05:49] -> Part 1/1 - Run 5/6: Preparing Calibration Searches...
INFO:    [31/07/2026 12:05:50] -> Part 1/1 - Run 5/6: First Pass Calibration Search...
INFO:    [31/07/2026 12:06:05] -> Part 1/1 - Run 5/6: Calibration Search...
INFO:    [31/07/2026 12:06:11] -> The number of PSMs identified during calibration with FDR <= 0.01 is 5501 [6.4s]
INFO:    [31/07/2026 12:06:37] -> Part 1/1 - Run 5/6: Preparing Main Search...
INFO:    [31/07/2026 12:06:39] -> Part 1/1 - Run 5/6: Main Search...
INFO:    [31/07/2026 12:10:09] -> Extracting 419332 MS1 XICs
INFO:    [31/07/2026 12:15:17] -> Run 5/6: Score Post-Processing
INFO:    [31/07/2026 12:17:29] -> Run 5/6: Cleaning Up Run...
INFO:    [31/07/2026 12:17:33] -> SuperRun 6/6: Initializing (LFQ_Astral_DIA_15min_50ng_Condition_B_REP3.raw)
INFO:    [31/07/2026 12:17:33] -> Run 6/6: Organizing Data from Run
INFO:    [31/07/2026 12:17:33] -> Run 6/6: Creating Calibration Search Space...
INFO:    [31/07/2026 12:17:33] -> Run 6/6: Creating Search Space...
INFO:    [31/07/2026 12:17:33] -> Run 6/6: MS2 Index Generation
INFO:    [31/07/2026 12:17:35] -> MS2 Index Generation Time: 1.35s
INFO:    [31/07/2026 12:17:35] -> Run 6/6: Method-Specific Pre-Processing...
INFO:    [31/07/2026 12:17:41] -> Initializing...
INFO:    [31/07/2026 12:18:26] -> Finalizing Scan Map...
INFO:    [31/07/2026 12:19:51] -> Processed in 2.2m
INFO:    [31/07/2026 12:19:57] -> Done
INFO:    [31/07/2026 12:20:04] -> Run 6/6: Process Raw File...
INFO:    [31/07/2026 12:20:09] -> Run 6/6: Preparing Partitions...
INFO:    [31/07/2026 12:20:09] -> Part 1/1 - Run 6/6: Preparing Calibration Searches...
INFO:    [31/07/2026 12:20:11] -> Part 1/1 - Run 6/6: First Pass Calibration Search...
INFO:    [31/07/2026 12:20:25] -> Part 1/1 - Run 6/6: Calibration Search...
INFO:    [31/07/2026 12:20:32] -> The number of PSMs identified during calibration with FDR <= 0.01 is 6194 [6.5s]
INFO:    [31/07/2026 12:21:00] -> Part 1/1 - Run 6/6: Preparing Main Search...
INFO:    [31/07/2026 12:21:01] -> Part 1/1 - Run 6/6: Main Search...
INFO:    [31/07/2026 12:23:27] -> Extracting 420524 MS1 XICs
INFO:    [31/07/2026 12:28:29] -> Run 6/6: Score Post-Processing
INFO:    [31/07/2026 12:31:34] -> Run 6/6: Cleaning Up Run...
INFO:    [31/07/2026 12:31:40] -> Remove Aborted Runs (if any) from the Experiment...
INFO:    [31/07/2026 12:31:40] -> PSM FDR...
INFO:    [31/07/2026 12:31:41] -> PSM FDR: 525.94ms
INFO:    [31/07/2026 12:31:41] -> Converting to non-redundant data structure...
INFO:    [31/07/2026 12:32:03] -> Performing Peptide FDR...
INFO:    [31/07/2026 12:32:08] -> Performing Protein Inference...
INFO:    [31/07/2026 12:32:11] -> Performing Protein FDR...
INFO:    [31/07/2026 12:32:14] -> Calculating Result Values at Run and Experiment Level...
INFO:    [31/07/2026 12:32:28] -> Pulsar identified 324985 PSMs, 71514 stripped sequences, 79744 peptide precursors, 7711 protein groups.
INFO:    [31/07/2026 12:32:28] -> Annotating In-Source Fragmentation...
INFO:    [31/07/2026 12:32:28] -> iRT Calibration...
INFO:    [31/07/2026 12:32:33] -> Performing directDIA+ search fine tuning...
INFO:    [31/07/2026 12:42:27] -> Performing directDIA+ search...
INFO:    [31/07/2026 12:42:27] -> directDIA+ search: LFQ_Astral_DIA_15min_50ng_Condition_A_REP1.raw
INFO:    [31/07/2026 12:54:56] -> directDIA+ search: LFQ_Astral_DIA_15min_50ng_Condition_A_REP2.raw
INFO:    [31/07/2026 13:00:47] -> directDIA+ search: LFQ_Astral_DIA_15min_50ng_Condition_A_REP3.raw
INFO:    [31/07/2026 13:05:32] -> directDIA+ search: LFQ_Astral_DIA_15min_50ng_Condition_B_REP1.raw
INFO:    [31/07/2026 13:13:22] -> directDIA+ search: LFQ_Astral_DIA_15min_50ng_Condition_B_REP2.raw
INFO:    [31/07/2026 13:18:02] -> directDIA+ search: LFQ_Astral_DIA_15min_50ng_Condition_B_REP3.raw
INFO:    [31/07/2026 13:23:23] -> Total nr of Searched Peptides: 1 328 279 of 1 960 544
INFO:    [31/07/2026 13:23:26] -> Initializing Combined Protein Inference...
INFO:    [31/07/2026 13:32:41] -> Performing Protein Inference...
INFO:    [31/07/2026 13:32:52] -> Performing Protein FDR...
INFO:    [31/07/2026 13:32:55] -> Calculating Result Values at Run and Experiment Level...
INFO:    [31/07/2026 13:33:09] -> Pulsar identified 324957 PSMs, 71486 stripped sequences, 79716 peptide precursors, 7717 protein groups.
INFO:    [31/07/2026 13:33:13] -> Calculating Summary...
INFO:    [31/07/2026 13:33:17] -> Identifying Calibration Peptides...
INFO:    [31/07/2026 13:33:18] -> Performing directDIA+ Post Processing...
INFO:    [31/07/2026 13:35:13] -> Combining Protein-Group FDR Results...
INFO:    [31/07/2026 13:35:13] -> Combined Protein-Group Identifications at 1% Qvalue: 12 346
INFO:    [31/07/2026 13:35:14] -> Writing experiment store..
INFO:    [31/07/2026 13:35:35] -> Assigning iRT Source...
INFO:    [31/07/2026 13:35:35] -> Calculating iRT...
INFO:    [31/07/2026 13:35:36] -> Cleaning Up Experiment...
INFO:    [31/07/2026 13:35:36] -> Summarizing Identifications
INFO:    [31/07/2026 13:36:56] -> Predicting Ion Mobility
INFO:    [31/07/2026 13:37:52] -> Calculating Median iRT
INFO:    [31/07/2026 13:37:55] -> Building BGS Protein Grouping...
INFO:    [31/07/2026 13:37:56] -> Digesting Fasta...
INFO:    [31/07/2026 13:37:56] -> Annotating Proteins...
INFO:    [31/07/2026 13:37:57] -> Grouping Proteins...
INFO:    [31/07/2026 13:38:00] -> Calculating Run Summary Statistics...
INFO:    [31/07/2026 13:38:09] -> Building Consensus Fragment Spectra...
INFO:    [31/07/2026 13:38:39] -> Selecting best fragment ions
INFO:    [31/07/2026 13:38:39] -> Condensing Global Results...
INFO:    [31/07/2026 13:39:51] -> Protein Groups: 11500 (11592), Protein Id: 11666 (11776), Modified Peptides: 124729, Precursors: 140309, Peptides: 122855, Fragments: 831053
INFO:    [31/07/2026 13:40:30] -> Initializing Experiment...
INFO:    [31/07/2026 13:40:30] -> Loading Spectral Libraries...
INFO:    [31/07/2026 14:13:44] -> Initialize Scoring...
INFO:    [31/07/2026 14:13:44] -> Generating Scan Map...
INFO:    [31/07/2026 14:13:44] -> Initializing Workpackages...
INFO:    [31/07/2026 14:13:44] -> Performing Basic Calibration...
INFO:    [31/07/2026 14:13:45] -> Calibration successful
INFO:    [31/07/2026 14:13:45] -> Identifying Calibration Peptides...
INFO:    [31/07/2026 14:13:45] -> Calibration successful
INFO:    [31/07/2026 14:13:45] -> Correcting Gradient Fine Structure...
INFO:    [31/07/2026 14:13:49] -> Initializing Pipeline...
INFO:    [31/07/2026 14:13:49] -> Preprocessing Run #1 of 6...
INFO:    [31/07/2026 14:13:49] -> Initialize Scoring...
INFO:    [31/07/2026 14:13:49] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:13:58] -> Machine Learning...
INFO:    [31/07/2026 14:14:00] -> Pipeline executed in 10.62s - Current Experiment had 0 Warnings and 0 Errors.
INFO:    [31/07/2026 14:14:00] -> Calibration successful
INFO:    [31/07/2026 14:14:04] -> Initializing Pipeline...
INFO:    [31/07/2026 14:14:04] -> Preprocessing Run #1 of 6...
INFO:    [31/07/2026 14:14:04] -> Initialize Scoring...
INFO:    [31/07/2026 14:14:04] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:14:24] -> Machine Learning...
INFO:    [31/07/2026 14:14:31] -> Pipeline executed in 26.98s - Current Experiment had 0 Warnings and 0 Errors.
INFO:    [31/07/2026 14:14:36] -> Calibration successful
INFO:    [31/07/2026 14:14:36] -> Initialize Scoring...
INFO:    [31/07/2026 14:14:36] -> Preprocessing Run #2 of 6...
INFO:    [31/07/2026 14:14:36] -> Searching DIA with Pulsar...
INFO:    [31/07/2026 14:14:36] -> Initializing Experiment...
INFO:    [31/07/2026 14:14:36] -> Loading Spectral Libraries...
INFO:    [31/07/2026 14:14:36] -> Initialize Scoring...
INFO:    [31/07/2026 14:14:36] -> Generating Scan Map...
INFO:    [31/07/2026 14:14:36] -> Initializing Workpackages...
INFO:    [31/07/2026 14:14:36] -> Performing Basic Calibration...
INFO:    [31/07/2026 14:14:37] -> Calibration successful
INFO:    [31/07/2026 14:14:37] -> Identifying Calibration Peptides...
INFO:    [31/07/2026 14:14:37] -> Calibration successful
INFO:    [31/07/2026 14:14:37] -> Correcting Gradient Fine Structure...
INFO:    [31/07/2026 14:14:41] -> Initializing Pipeline...
INFO:    [31/07/2026 14:14:41] -> Preprocessing Run #2 of 6...
INFO:    [31/07/2026 14:14:41] -> Initialize Scoring...
INFO:    [31/07/2026 14:14:41] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:14:48] -> Machine Learning...
INFO:    [31/07/2026 14:14:49] -> Pipeline executed in 8.8s - Current Experiment had 0 Warnings and 0 Errors.
INFO:    [31/07/2026 14:14:50] -> Calibration successful
INFO:    [31/07/2026 14:14:54] -> Initializing Pipeline...
INFO:    [31/07/2026 14:14:54] -> Preprocessing Run #2 of 6...
INFO:    [31/07/2026 14:14:54] -> Initialize Scoring...
INFO:    [31/07/2026 14:14:54] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:15:14] -> Machine Learning...
INFO:    [31/07/2026 14:15:20] -> Pipeline executed in 26.77s - Current Experiment had 0 Warnings and 0 Errors.
INFO:    [31/07/2026 14:15:25] -> Calibration successful
INFO:    [31/07/2026 14:15:25] -> Initialize Scoring...
INFO:    [31/07/2026 14:15:25] -> Preprocessing Run #3 of 6...
INFO:    [31/07/2026 14:15:25] -> Searching DIA with Pulsar...
INFO:    [31/07/2026 14:15:25] -> Initializing Experiment...
INFO:    [31/07/2026 14:15:25] -> Loading Spectral Libraries...
INFO:    [31/07/2026 14:15:25] -> Initialize Scoring...
INFO:    [31/07/2026 14:15:26] -> Generating Scan Map...
INFO:    [31/07/2026 14:15:26] -> Initializing Workpackages...
INFO:    [31/07/2026 14:15:26] -> Performing Basic Calibration...
INFO:    [31/07/2026 14:15:26] -> Calibration successful
INFO:    [31/07/2026 14:15:26] -> Identifying Calibration Peptides...
INFO:    [31/07/2026 14:15:26] -> Calibration successful
INFO:    [31/07/2026 14:15:26] -> Correcting Gradient Fine Structure...
INFO:    [31/07/2026 14:15:30] -> Initializing Pipeline...
INFO:    [31/07/2026 14:15:30] -> Preprocessing Run #3 of 6...
INFO:    [31/07/2026 14:15:30] -> Initialize Scoring...
INFO:    [31/07/2026 14:15:30] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:15:37] -> Machine Learning...
INFO:    [31/07/2026 14:15:38] -> Pipeline executed in 8.7s - Current Experiment had 0 Warnings and 0 Errors.
INFO:    [31/07/2026 14:15:39] -> Calibration successful
INFO:    [31/07/2026 14:15:43] -> Initializing Pipeline...
INFO:    [31/07/2026 14:15:43] -> Preprocessing Run #3 of 6...
INFO:    [31/07/2026 14:15:43] -> Initialize Scoring...
INFO:    [31/07/2026 14:15:43] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:16:03] -> Machine Learning...
INFO:    [31/07/2026 14:16:09] -> Pipeline executed in 26.58s - Current Experiment had 0 Warnings and 0 Errors.
INFO:    [31/07/2026 14:16:18] -> Calibration successful
INFO:    [31/07/2026 14:16:18] -> Initialize Scoring...
INFO:    [31/07/2026 14:16:18] -> Preprocessing Run #4 of 6...
INFO:    [31/07/2026 14:16:18] -> Searching DIA with Pulsar...
INFO:    [31/07/2026 14:16:18] -> Initializing Experiment...
INFO:    [31/07/2026 14:16:18] -> Loading Spectral Libraries...
INFO:    [31/07/2026 14:16:18] -> Initialize Scoring...
INFO:    [31/07/2026 14:16:18] -> Generating Scan Map...
INFO:    [31/07/2026 14:16:18] -> Initializing Workpackages...
INFO:    [31/07/2026 14:16:18] -> Performing Basic Calibration...
INFO:    [31/07/2026 14:16:18] -> Calibration successful
INFO:    [31/07/2026 14:16:18] -> Identifying Calibration Peptides...
INFO:    [31/07/2026 14:16:18] -> Calibration successful
INFO:    [31/07/2026 14:16:18] -> Correcting Gradient Fine Structure...
INFO:    [31/07/2026 14:16:22] -> Initializing Pipeline...
INFO:    [31/07/2026 14:16:22] -> Preprocessing Run #4 of 6...
INFO:    [31/07/2026 14:16:22] -> Initialize Scoring...
INFO:    [31/07/2026 14:16:22] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:16:29] -> Machine Learning...
INFO:    [31/07/2026 14:16:30] -> Pipeline executed in 8.48s - Current Experiment had 0 Warnings and 0 Errors.
INFO:    [31/07/2026 14:16:31] -> Calibration successful
INFO:    [31/07/2026 14:16:35] -> Initializing Pipeline...
INFO:    [31/07/2026 14:16:35] -> Preprocessing Run #4 of 6...
INFO:    [31/07/2026 14:16:35] -> Initialize Scoring...
INFO:    [31/07/2026 14:16:35] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:16:55] -> Machine Learning...
INFO:    [31/07/2026 14:17:02] -> Pipeline executed in 27.04s - Current Experiment had 0 Warnings and 0 Errors.
INFO:    [31/07/2026 14:17:07] -> Calibration successful
INFO:    [31/07/2026 14:17:07] -> Initialize Scoring...
INFO:    [31/07/2026 14:17:07] -> Preprocessing Run #5 of 6...
INFO:    [31/07/2026 14:17:07] -> Searching DIA with Pulsar...
INFO:    [31/07/2026 14:17:07] -> Initializing Experiment...
INFO:    [31/07/2026 14:17:07] -> Loading Spectral Libraries...
INFO:    [31/07/2026 14:17:07] -> Initialize Scoring...
INFO:    [31/07/2026 14:17:07] -> Generating Scan Map...
INFO:    [31/07/2026 14:17:07] -> Initializing Workpackages...
INFO:    [31/07/2026 14:17:07] -> Performing Basic Calibration...
INFO:    [31/07/2026 14:17:07] -> Calibration successful
INFO:    [31/07/2026 14:17:07] -> Identifying Calibration Peptides...
INFO:    [31/07/2026 14:17:07] -> Calibration successful
INFO:    [31/07/2026 14:17:07] -> Correcting Gradient Fine Structure...
INFO:    [31/07/2026 14:17:12] -> Initializing Pipeline...
INFO:    [31/07/2026 14:17:12] -> Preprocessing Run #5 of 6...
INFO:    [31/07/2026 14:17:12] -> Initialize Scoring...
INFO:    [31/07/2026 14:17:12] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:17:19] -> Machine Learning...
INFO:    [31/07/2026 14:17:20] -> Pipeline executed in 8.61s - Current Experiment had 0 Warnings and 0 Errors.
INFO:    [31/07/2026 14:17:21] -> Calibration successful
INFO:    [31/07/2026 14:17:25] -> Initializing Pipeline...
INFO:    [31/07/2026 14:17:25] -> Preprocessing Run #5 of 6...
INFO:    [31/07/2026 14:17:25] -> Initialize Scoring...
INFO:    [31/07/2026 14:17:25] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:17:45] -> Machine Learning...
INFO:    [31/07/2026 14:17:51] -> Pipeline executed in 26.18s - Current Experiment had 0 Warnings and 0 Errors.
INFO:    [31/07/2026 14:17:56] -> Calibration successful
INFO:    [31/07/2026 14:17:56] -> Initialize Scoring...
INFO:    [31/07/2026 14:17:56] -> Preprocessing Run #6 of 6...
INFO:    [31/07/2026 14:17:56] -> Searching DIA with Pulsar...
INFO:    [31/07/2026 14:17:56] -> Initializing Experiment...
INFO:    [31/07/2026 14:17:56] -> Loading Spectral Libraries...
INFO:    [31/07/2026 14:17:56] -> Initialize Scoring...
INFO:    [31/07/2026 14:17:56] -> Generating Scan Map...
INFO:    [31/07/2026 14:17:56] -> Initializing Workpackages...
INFO:    [31/07/2026 14:17:56] -> Performing Basic Calibration...
INFO:    [31/07/2026 14:17:57] -> Calibration successful
INFO:    [31/07/2026 14:17:57] -> Identifying Calibration Peptides...
INFO:    [31/07/2026 14:17:57] -> Calibration successful
INFO:    [31/07/2026 14:17:57] -> Correcting Gradient Fine Structure...
INFO:    [31/07/2026 14:18:01] -> Initializing Pipeline...
INFO:    [31/07/2026 14:18:01] -> Preprocessing Run #6 of 6...
INFO:    [31/07/2026 14:18:01] -> Initialize Scoring...
INFO:    [31/07/2026 14:18:01] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:18:08] -> Machine Learning...
INFO:    [31/07/2026 14:18:09] -> Pipeline executed in 8.55s - Current Experiment had 0 Warnings and 0 Errors.
INFO:    [31/07/2026 14:18:13] -> Calibration successful
INFO:    [31/07/2026 14:18:18] -> Initializing Pipeline...
INFO:    [31/07/2026 14:18:18] -> Preprocessing Run #6 of 6...
INFO:    [31/07/2026 14:18:18] -> Initialize Scoring...
INFO:    [31/07/2026 14:18:18] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:18:38] -> Machine Learning...
INFO:    [31/07/2026 14:18:45] -> Pipeline executed in 27.13s - Current Experiment had 0 Warnings and 0 Errors.
INFO:    [31/07/2026 14:18:50] -> Calibration successful
INFO:    [31/07/2026 14:18:50] -> Initialize Scoring...
INFO:    [31/07/2026 14:18:50] -> Initializing QC...
INFO:    [31/07/2026 14:18:50] -> Determining Calibration Parameter...
INFO:    [31/07/2026 14:18:50] -> Assigning ML Features...
INFO:    [31/07/2026 14:18:51] -> Generating Decoys...
INFO:    [31/07/2026 14:28:25] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:32:21] -> Releasing Raw Files...
INFO:    [31/07/2026 14:32:21] -> Machine Learning...
INFO:    [31/07/2026 14:34:59] -> Initializing HTRMS...
INFO:    [31/07/2026 14:34:59] -> Releasing Run Resources...
INFO:    [31/07/2026 14:35:00] -> Reducing Score Cache...
INFO:    [31/07/2026 14:35:08] -> Calculating Qvalues...
INFO:    [31/07/2026 14:35:11] -> Unique precursors: 131 875 of 140 309 | modified peptides: 118 477 of 124 729 | peptides: 116 667 of 122 855 |  protein groups: 11 245 (Qvalue <= 0.01) [LFQ_Astral_DIA_15min_50ng_Condition_A_REP1.raw]
INFO:    [31/07/2026 14:35:11] -> Calibration successful
INFO:    [31/07/2026 14:35:11] -> Annotating In-Source Fragmentation...
INFO:    [31/07/2026 14:35:12] -> Collapsing Search Tree...
INFO:    [31/07/2026 14:35:12] -> Processed in 16.36m
INFO:    [31/07/2026 14:35:12] -> Initializing QC...
INFO:    [31/07/2026 14:35:12] -> Determining Calibration Parameter...
INFO:    [31/07/2026 14:35:12] -> Assigning ML Features...
INFO:    [31/07/2026 14:35:13] -> Generating Decoys...
INFO:    [31/07/2026 14:35:13] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:39:23] -> Releasing Raw Files...
INFO:    [31/07/2026 14:39:23] -> Machine Learning...
INFO:    [31/07/2026 14:42:47] -> Initializing HTRMS...
INFO:    [31/07/2026 14:42:47] -> Releasing Run Resources...
INFO:    [31/07/2026 14:42:48] -> Reducing Score Cache...
INFO:    [31/07/2026 14:42:59] -> Calculating Qvalues...
INFO:    [31/07/2026 14:43:02] -> Unique precursors: 132 139 of 140 309 | modified peptides: 118 683 of 124 729 | peptides: 116 849 of 122 855 |  protein groups: 11 193 (Qvalue <= 0.01) [LFQ_Astral_DIA_15min_50ng_Condition_A_REP2.raw]
INFO:    [31/07/2026 14:43:02] -> Calibration successful
INFO:    [31/07/2026 14:43:02] -> Annotating In-Source Fragmentation...
INFO:    [31/07/2026 14:43:03] -> Collapsing Search Tree...
INFO:    [31/07/2026 14:43:03] -> Processed in 7.85m
INFO:    [31/07/2026 14:43:04] -> Initializing QC...
INFO:    [31/07/2026 14:43:04] -> Determining Calibration Parameter...
INFO:    [31/07/2026 14:43:05] -> Assigning ML Features...
INFO:    [31/07/2026 14:43:05] -> Generating Decoys...
INFO:    [31/07/2026 14:43:05] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:47:28] -> Releasing Raw Files...
INFO:    [31/07/2026 14:47:28] -> Machine Learning...
INFO:    [31/07/2026 14:49:52] -> Initializing HTRMS...
INFO:    [31/07/2026 14:49:52] -> Releasing Run Resources...
INFO:    [31/07/2026 14:49:52] -> Reducing Score Cache...
INFO:    [31/07/2026 14:50:00] -> Calculating Qvalues...
INFO:    [31/07/2026 14:50:04] -> Unique precursors: 133 272 of 140 309 | modified peptides: 119 545 of 124 729 | peptides: 117 716 of 122 855 |  protein groups: 11 239 (Qvalue <= 0.01) [LFQ_Astral_DIA_15min_50ng_Condition_A_REP3.raw]
INFO:    [31/07/2026 14:50:04] -> Calibration successful
INFO:    [31/07/2026 14:50:04] -> Annotating In-Source Fragmentation...
INFO:    [31/07/2026 14:50:05] -> Collapsing Search Tree...
INFO:    [31/07/2026 14:50:05] -> Processed in 7.01m
INFO:    [31/07/2026 14:50:05] -> Initializing QC...
INFO:    [31/07/2026 14:50:05] -> Determining Calibration Parameter...
INFO:    [31/07/2026 14:50:05] -> Assigning ML Features...
INFO:    [31/07/2026 14:50:06] -> Generating Decoys...
INFO:    [31/07/2026 14:50:06] -> Extracting Ion Currents...
INFO:    [31/07/2026 14:54:01] -> Releasing Raw Files...
INFO:    [31/07/2026 14:54:01] -> Machine Learning...
INFO:    [31/07/2026 14:56:27] -> Initializing HTRMS...
INFO:    [31/07/2026 14:56:27] -> Releasing Run Resources...
INFO:    [31/07/2026 14:56:28] -> Reducing Score Cache...
INFO:    [31/07/2026 14:56:37] -> Calculating Qvalues...
INFO:    [31/07/2026 14:56:39] -> Unique precursors: 133 278 of 140 309 | modified peptides: 119 574 of 124 729 | peptides: 117 710 of 122 855 |  protein groups: 11 241 (Qvalue <= 0.01) [LFQ_Astral_DIA_15min_50ng_Condition_B_REP1.raw]
INFO:    [31/07/2026 14:56:39] -> Calibration successful
INFO:    [31/07/2026 14:56:39] -> Annotating In-Source Fragmentation...
INFO:    [31/07/2026 14:56:40] -> Collapsing Search Tree...
INFO:    [31/07/2026 14:56:40] -> Processed in 6.59m
INFO:    [31/07/2026 14:56:41] -> Initializing QC...
INFO:    [31/07/2026 14:56:41] -> Determining Calibration Parameter...
INFO:    [31/07/2026 14:56:41] -> Assigning ML Features...
INFO:    [31/07/2026 14:56:41] -> Generating Decoys...
INFO:    [31/07/2026 14:56:42] -> Extracting Ion Currents...
INFO:    [31/07/2026 15:00:40] -> Releasing Raw Files...
INFO:    [31/07/2026 15:00:40] -> Machine Learning...
INFO:    [31/07/2026 15:02:56] -> Initializing HTRMS...
INFO:    [31/07/2026 15:02:56] -> Releasing Run Resources...
INFO:    [31/07/2026 15:02:57] -> Reducing Score Cache...
INFO:    [31/07/2026 15:03:05] -> Calculating Qvalues...
INFO:    [31/07/2026 15:03:07] -> Unique precursors: 133 176 of 140 309 | modified peptides: 119 525 of 124 729 | peptides: 117 662 of 122 855 |  protein groups: 11 239 (Qvalue <= 0.01) [LFQ_Astral_DIA_15min_50ng_Condition_B_REP2.raw]
INFO:    [31/07/2026 15:03:07] -> Calibration successful
INFO:    [31/07/2026 15:03:08] -> Annotating In-Source Fragmentation...
INFO:    [31/07/2026 15:03:08] -> Collapsing Search Tree...
INFO:    [31/07/2026 15:03:09] -> Processed in 6.47m
INFO:    [31/07/2026 15:03:09] -> Initializing QC...
INFO:    [31/07/2026 15:03:09] -> Determining Calibration Parameter...
INFO:    [31/07/2026 15:03:09] -> Assigning ML Features...
INFO:    [31/07/2026 15:03:09] -> Generating Decoys...
INFO:    [31/07/2026 15:03:10] -> Extracting Ion Currents...
INFO:    [31/07/2026 15:07:26] -> Releasing Raw Files...
INFO:    [31/07/2026 15:07:26] -> Machine Learning...
INFO:    [31/07/2026 15:09:53] -> Initializing HTRMS...
INFO:    [31/07/2026 15:09:53] -> Releasing Run Resources...
INFO:    [31/07/2026 15:09:54] -> Reducing Score Cache...
INFO:    [31/07/2026 15:10:02] -> Calculating Qvalues...
INFO:    [31/07/2026 15:10:05] -> Unique precursors: 133 330 of 140 309 | modified peptides: 119 603 of 124 729 | peptides: 117 738 of 122 855 |  protein groups: 11 234 (Qvalue <= 0.01) [LFQ_Astral_DIA_15min_50ng_Condition_B_REP3.raw]
INFO:    [31/07/2026 15:10:05] -> Calibration successful
INFO:    [31/07/2026 15:10:05] -> Annotating In-Source Fragmentation...
INFO:    [31/07/2026 15:10:06] -> Collapsing Search Tree...
INFO:    [31/07/2026 15:10:06] -> Processed in 6.95m
INFO:    [31/07/2026 15:10:06] -> Normalizing Cscores...
INFO:    [31/07/2026 15:10:07] -> Calculating Global CVs
INFO:    [31/07/2026 15:10:09] -> Calculating Profile Qvalues...
INFO:    [31/07/2026 15:10:12] -> Initializing Protein Groups...
INFO:    [31/07/2026 15:10:45] -> Correcting Interferences...
INFO:    [31/07/2026 15:11:29] -> Calculating Global CVs
INFO:    [31/07/2026 15:11:30] -> Calculating Precursor Quantities...
INFO:    [31/07/2026 15:11:30] -> Normalizing Quantification...
INFO:    [31/07/2026 15:11:34] -> Performing Local Normalization...
INFO:    [31/07/2026 15:11:37] -> Calculating Global CVs
INFO:    [31/07/2026 15:11:38] -> Calculating Condition CVs
INFO:    [31/07/2026 15:11:43] -> Excluding Library Duplicates...
INFO:    [31/07/2026 15:11:44] -> Calculating Global CVs
INFO:    [31/07/2026 15:11:46] -> Building Protein Groups...
INFO:    [31/07/2026 15:12:17] -> Calculating Run-Wise Protein Group FDR...
INFO:    [31/07/2026 15:12:23] -> Annotating Protein Single Hits...
INFO:    [31/07/2026 15:12:26] -> Calculating Global CVs
INFO:    [31/07/2026 15:12:27] -> Updating Identification Counts...
INFO:    [31/07/2026 15:12:34] -> Subtracting background noise...
INFO:    [31/07/2026 15:12:34] -> Resetting existing imputation...
INFO:    [31/07/2026 15:12:34] -> Calculating Protein Quantities...
INFO:    [31/07/2026 15:12:36] -> Calculating Protein Quantities...
INFO:    [31/07/2026 15:12:36] -> Calculating MaxLFQ Protein Quantities...
INFO:    [31/07/2026 15:12:49] -> Collapsing PTM-Locations...
INFO:    [31/07/2026 15:12:49] -> PTM Stoichiometry calculation...
INFO:    [31/07/2026 15:12:49] -> Creating Protein Map...
INFO:    [31/07/2026 15:12:49] -> Compiling Run Summary Information...
INFO:    [31/07/2026 15:12:50] -> Running Post Analysis Processes...
INFO:    [31/07/2026 15:13:37] -> Saving Qc Data...
INFO:    [31/07/2026 15:13:44] -> Generating Output
INFO:    [31/07/2026 15:13:44] -> Generating Experiment Report
INFO:    [31/07/2026 15:15:28] -> 20260831_Quant_Astral_DIA_forRobbe_Report_BGS Factory Report (Normal).tsv
INFO:    [31/07/2026 15:15:28] -> Generating RT Recalibration Report
INFO:    [31/07/2026 15:15:28] -> LFQ_Astral_DIA_15min_50ng_Condition_A_REP1.raw_1_iRTCalibration.tsv
INFO:    [31/07/2026 15:15:28] -> LFQ_Astral_DIA_15min_50ng_Condition_A_REP2.raw_2_iRTCalibration.tsv
INFO:    [31/07/2026 15:15:28] -> LFQ_Astral_DIA_15min_50ng_Condition_A_REP3.raw_3_iRTCalibration.tsv
INFO:    [31/07/2026 15:15:28] -> LFQ_Astral_DIA_15min_50ng_Condition_B_REP1.raw_4_iRTCalibration.tsv
INFO:    [31/07/2026 15:15:28] -> LFQ_Astral_DIA_15min_50ng_Condition_B_REP2.raw_5_iRTCalibration.tsv
INFO:    [31/07/2026 15:15:28] -> LFQ_Astral_DIA_15min_50ng_Condition_B_REP3.raw_6_iRTCalibration.tsv
INFO:    [31/07/2026 15:15:28] -> Generating Run Meta Report
INFO:    [31/07/2026 15:15:30] -> LFQ_Astral_DIA_15min_50ng_Condition_A_REP1.raw_1_RunOverview.tsv
INFO:    [31/07/2026 15:15:30] -> LFQ_Astral_DIA_15min_50ng_Condition_A_REP2.raw_2_RunOverview.tsv
INFO:    [31/07/2026 15:15:30] -> LFQ_Astral_DIA_15min_50ng_Condition_A_REP3.raw_3_RunOverview.tsv
INFO:    [31/07/2026 15:15:30] -> LFQ_Astral_DIA_15min_50ng_Condition_B_REP1.raw_4_RunOverview.tsv
INFO:    [31/07/2026 15:15:30] -> LFQ_Astral_DIA_15min_50ng_Condition_B_REP2.raw_5_RunOverview.tsv
INFO:    [31/07/2026 15:15:30] -> LFQ_Astral_DIA_15min_50ng_Condition_B_REP3.raw_6_RunOverview.tsv
INFO:    [31/07/2026 15:15:30] -> Generating Post Analysis Reports
INFO:    [31/07/2026 15:15:31] -> 20260831_Quant_Astral_DIA_forRobbe_AnalyisOverview.txt
INFO:    [31/07/2026 15:15:31] -> ProteinFDR-Histogram.pdf
INFO:    [31/07/2026 15:15:31] -> Heatmap.png
INFO:    [31/07/2026 15:15:31] -> Generating Normalization Report
INFO:    [31/07/2026 15:15:35] -> 20260831_Quant_Astral_DIA_forRobbe_Normalization.pdf
INFO:    [31/07/2026 15:15:35] -> Generating Condition Setup Report
INFO:    [31/07/2026 15:15:35] -> 20260831_Quant_Astral_DIA_forRobbe_ConditionSetup.tsv
INFO:    [31/07/2026 15:15:35] -> Generating Memory Consumption Report
INFO:    [31/07/2026 15:15:35] -> 20260831_Quant_Astral_DIA_forRobbe_MemoryConsumption.tsv
INFO:    [31/07/2026 15:15:35] -> Generating Experiment Settings Report
INFO:    [31/07/2026 15:15:37] -> 20260831_Quant_Astral_DIA_forRobbe_ExperimentSetupOverview_BGS Factory Settings.txt
INFO:    [31/07/2026 15:15:37] -> Writing Summary Information...
INFO:    [31/07/2026 15:15:37] -> Unique precursors: 131 822 of 140 309 | modified peptides: 118 424 of 124 729 | peptides: 116 614 of 122 855 |  protein groups: 11 193 (Qvalue <= 0.01) for run #1 "LFQ_Astral_DIA_15min_50ng_Condition_A_REP1.raw"
INFO:    [31/07/2026 15:15:37] -> Unique precursors: 132 105 of 140 309 | modified peptides: 118 649 of 124 729 | peptides: 116 815 of 122 855 |  protein groups: 11 161 (Qvalue <= 0.01) for run #2 "LFQ_Astral_DIA_15min_50ng_Condition_A_REP2.raw"
INFO:    [31/07/2026 15:15:37] -> Unique precursors: 133 211 of 140 309 | modified peptides: 119 484 of 124 729 | peptides: 117 655 of 122 855 |  protein groups: 11 183 (Qvalue <= 0.01) for run #3 "LFQ_Astral_DIA_15min_50ng_Condition_A_REP3.raw"
INFO:    [31/07/2026 15:15:37] -> Unique precursors: 133 236 of 140 309 | modified peptides: 119 532 of 124 729 | peptides: 117 668 of 122 855 |  protein groups: 11 202 (Qvalue <= 0.01) for run #4 "LFQ_Astral_DIA_15min_50ng_Condition_B_REP1.raw"
INFO:    [31/07/2026 15:15:37] -> Unique precursors: 133 141 of 140 309 | modified peptides: 119 490 of 124 729 | peptides: 117 627 of 122 855 |  protein groups: 11 205 (Qvalue <= 0.01) for run #5 "LFQ_Astral_DIA_15min_50ng_Condition_B_REP2.raw"
INFO:    [31/07/2026 15:15:37] -> Unique precursors: 133 290 of 140 309 | modified peptides: 119 563 of 124 729 | peptides: 117 698 of 122 855 |  protein groups: 11 196 (Qvalue <= 0.01) for run #6 "LFQ_Astral_DIA_15min_50ng_Condition_B_REP3.raw"
INFO:    [31/07/2026 15:15:37] -> Unique precursors: 140 166 of 140 309 | modified peptides: 124 659 of 124 729 | peptides: 122 785 of 122 855 |  protein groups: 11 460 (Qvalue <= 0.01) for whole experiment "20260831_Quant_Astral_DIA_forRobbe"

INFO:    [31/07/2026 15:15:37] -> Saving SNE File...
INFO:    [31/07/2026 15:15:37] -> Storing SNE File: D:\Utilisateurs\locard\SpectronautFiles\20260831_ProteoBench_DIAQuantAstralModule\20260731_100134_20260831_Quant_Astral_DIA_forRobbe\20260731_100134_20260831_Quant_Astral_DIA_forRobbe.sne
INFO:    [31/07/2026 15:15:37] -> Initializing Pipeline...
INFO:    [31/07/2026 15:15:37] -> Calculating Qvalues...
INFO:    [31/07/2026 15:15:38] -> Saving...
INFO:    [31/07/2026 16:30:57] -> Applying settings to loaded experiment...
INFO:    [31/07/2026 16:30:57] -> Updating the condition setup for the experiment
INFO:    [31/07/2026 16:30:57] -> Updating Quantities...
INFO:    [31/07/2026 16:30:57] -> Unifying Meta...
INFO:    [31/07/2026 16:30:58] -> Normalizing Cscores...
INFO:    [31/07/2026 16:30:59] -> Calculating Global CVs
INFO:    [31/07/2026 16:31:04] -> Calculating Profile Qvalues...
INFO:    [31/07/2026 16:31:08] -> Linking References...
INFO:    [31/07/2026 16:31:10] -> Initializing Protein Groups...
INFO:    [31/07/2026 16:31:26] -> Building Protein Groups...
INFO:    [31/07/2026 16:31:33] -> Annotating In-Source Fragmentation...
INFO:    [31/07/2026 16:31:33] -> Calculating Run-Wise Protein Group FDR...
INFO:    [31/07/2026 16:31:47] -> Annotating Protein Single Hits...
INFO:    [31/07/2026 16:31:49] -> Normalizing Quantification...
INFO:    [31/07/2026 16:31:50] -> Calculating Global CVs
INFO:    [31/07/2026 16:31:51] -> Updating Identification Counts...
INFO:    [31/07/2026 16:31:55] -> Subtracting background noise...
INFO:    [31/07/2026 16:31:56] -> Resetting existing imputation...
INFO:    [31/07/2026 16:31:56] -> Calculating Protein Quantities...
INFO:    [31/07/2026 16:32:01] -> Calculating Protein Quantities...
INFO:    [31/07/2026 16:32:01] -> Calculating MaxLFQ Protein Quantities...
INFO:    [31/07/2026 16:32:15] -> Collapsing PTM-Locations...
INFO:    [31/07/2026 16:32:15] -> PTM Stoichiometry calculation...
INFO:    [31/07/2026 16:32:15] -> Calculating Condition CVs
INFO:    [31/07/2026 16:32:23] -> Creating Protein Map...
INFO:    [31/07/2026 16:32:23] -> Compiling Run Summary Information...
INFO:    [31/07/2026 16:32:24] -> Running Post Analysis Processes...
INFO:    [31/07/2026 16:42:00] -> Writing Summary Information...
INFO:    [31/07/2026 16:42:00] -> Unique precursors: 131 822 of 140 309 | modified peptides: 118 424 of 124 729 | peptides: 116 614 of 122 855 |  protein groups: 11 193 (Qvalue <= 0.01) for run #1 "LFQ_Astral_DIA_15min_50ng_Condition_A_REP1.raw"
INFO:    [31/07/2026 16:42:00] -> Unique precursors: 132 105 of 140 309 | modified peptides: 118 649 of 124 729 | peptides: 116 815 of 122 855 |  protein groups: 11 161 (Qvalue <= 0.01) for run #2 "LFQ_Astral_DIA_15min_50ng_Condition_A_REP2.raw"
INFO:    [31/07/2026 16:42:00] -> Unique precursors: 133 211 of 140 309 | modified peptides: 119 484 of 124 729 | peptides: 117 655 of 122 855 |  protein groups: 11 183 (Qvalue <= 0.01) for run #3 "LFQ_Astral_DIA_15min_50ng_Condition_A_REP3.raw"
INFO:    [31/07/2026 16:42:00] -> Unique precursors: 133 236 of 140 309 | modified peptides: 119 532 of 124 729 | peptides: 117 668 of 122 855 |  protein groups: 11 202 (Qvalue <= 0.01) for run #4 "LFQ_Astral_DIA_15min_50ng_Condition_B_REP1.raw"
INFO:    [31/07/2026 16:42:00] -> Unique precursors: 133 141 of 140 309 | modified peptides: 119 490 of 124 729 | peptides: 117 627 of 122 855 |  protein groups: 11 205 (Qvalue <= 0.01) for run #5 "LFQ_Astral_DIA_15min_50ng_Condition_B_REP2.raw"
INFO:    [31/07/2026 16:42:00] -> Unique precursors: 133 290 of 140 309 | modified peptides: 119 563 of 124 729 | peptides: 117 698 of 122 855 |  protein groups: 11 196 (Qvalue <= 0.01) for run #6 "LFQ_Astral_DIA_15min_50ng_Condition_B_REP3.raw"
INFO:    [31/07/2026 16:42:00] -> Unique precursors: 140 166 of 140 309 | modified peptides: 124 659 of 124 729 | peptides: 122 785 of 122 855 |  protein groups: 11 460 (Qvalue <= 0.01) for whole experiment "20260831_Quant_Astral_DIA_forRobbe"

INFO:    [31/07/2026 16:42:01] -> Current Experiment had 0 Warnings and 0 Errors.
INFO:    [31/07/2026 16:42:01] -> Pipeline executed in 11.93m - Current Experiment had 0 Warnings and 0 Errors.
[END-LOG]
