Metadata-Version: 2.4 Name: WhiskiWrap Version: 1.2.6 Summary: Whisk package wrapper created by Chris Rodgers, maintained by Vincent Prevosto Home-page: http://pypi.python.org/pypi/WhiskiWrap/ Author: Chris Rodgers, Ariel Iporre, Vincent Prevosto Author-email: Maintainer: vncntprvst License: The Janelia Farm Research Campus Software Copyright 1.1 Copyright (c) 2010, Howard Hughes Medical Institute, All rights reserved. Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: 1. Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. 2. Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. 3. Neither the name of the Howard Hughes Medical Institute nor the names of its contributors may be used to endorse or promote products derived from this software without specific prior written permission. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, ANY IMPLIED WARRANTIES OF MERCHANTABILITY, NON-INFRINGEMENT, OR FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; REASONABLE ROYALTIES; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. Project-URL: Homepage, http://pypi.python.org/pypi/WhiskiWrap/ Requires-Python: >=3.10 Description-Content-Type: text/markdown License-File: LICENSE.txt Requires-Dist: tables>=3.5.1 Requires-Dist: pandas Requires-Dist: MediaInfo Requires-Dist: future Requires-Dist: tifffile Requires-Dist: imagecodecs Requires-Dist: statsmodels Requires-Dist: ffmpeg-python>=0.2.0 Requires-Dist: whisk-janelia[ffmpeg] Requires-Dist: zarr Requires-Dist: pyarrow Requires-Dist: matplotlib Requires-Dist: easygui Requires-Dist: opencv-python Requires-Dist: h5py Requires-Dist: seaborn Requires-Dist: plotly Requires-Dist: psutil Requires-Dist: cmcrameri Requires-Dist: scikit-learn Requires-Dist: joblib Dynamic: home-page Dynamic: license-file Dynamic: requires-python # WhiskiWrap WhiskiWrap provides tools for running [whisk](https://github.com/vncntprvst/whisk) (the Janelia whisker tracker) more easily and efficiently. It improves on whisk by: 1. **Robust input** — it uses your system `ffmpeg` to read almost any video and to emit simple TIFF stacks that whisk traces reliably. 2. **Speed** — it runs many `trace` processes in parallel on non-overlapping chunks of the video. 3. **Portability / memory** — results are collected into HDF5 (or Parquet) files readable from Python or MATLAB, and can be read partially. The codebase is split into modules: `base` (core utilities), `pipeline` (high-level workflows), `io` (video I/O), and `wfile_io` / `mfile_io` (ctypes readers for whisk `.whiskers` / `.measurements` files). ## Installation **Requirements**: Python ≥ 3.10 and `ffmpeg` on your `PATH` (`ffmpeg -version` should run). Windows, Linux and macOS are supported. `whisk` is installed automatically as the `whisk-janelia` dependency — its prebuilt `trace`/`measure`/`classify` binaries (including **native Windows `.exe`s**) are bundled in the wheel, so no separate whisk install or `WHISKPATH` setup is needed. Install with [uv](https://docs.astral.sh/uv/) (recommended) or pip: ```bash # isolated environment uv venv source .venv/bin/activate # Windows: .venv\Scripts\activate uv pip install -e . # editable; or: uv pip install . ``` ```bash # plain pip pip install -e . # or: pip install whiskiwrap ``` Verify: ```bash python -c "import WhiskiWrap; print('ok')" ``` > Build tools are only needed if a dependency lacks a wheel for your platform > (Debian/Ubuntu: `python3-dev build-essential`; Fedora: `python3-devel gcc`; > macOS: `xcode-select --install`; Windows: usually none, since wheels are used). ## Quick start ```python import WhiskiWrap # Copy the input next to a working directory — many temporary files are created. input_video = 'test_video2.mp4' output_file = 'output.hdf5' # Trace (and optionally measure) in parallel chunks -> combined HDF5. WhiskiWrap.pipeline_trace(input_video, output_file, n_trace_processes=4) ``` Read the per-frame summary (tip/follicle/angle/… of every whisker): ```python import tables, pandas with tables.open_file(output_file) as fi: summary = pandas.DataFrame.from_records(fi.root.summary.read()) ``` For bilateral tracking with measurement per side, use `interleaved_split_trace_and_measure(...)` (see `WhiskiWrap/pipeline.py`), which crops each face side, traces in chunks, and writes a per-side Parquet/HDF5 file. ## How it works 1. Split the video into **epochs** (~100k frames) read into memory one at a time. 2. For each epoch: split into **chunks** (~1000 frames, optionally cropped), write each chunk as a TIFF stack, trace chunks with parallel `trace` instances, then append each chunk's `.whiskers` results to the output file. 3. Optionally delete intermediate chunk files. Key parameters: * `n_trace_processes` — parallel `trace` instances (≈ number of CPUs). * `epoch_sz_frames` — frames per epoch; as large as memory allows (e.g. 100000). * `chunk_sz_frames` — frames per chunk; ideally `epoch_sz_frames / (N * n_trace_processes)`. * `measure=True`, `face='left'|'right'` — also run `measure` for the given face side. ## Whisker linking & the learned add-on whisk identity classification (`classify`/HMM reclassify) only operates **within a chunk**. Preserving whisker identity **across chunks and across a whole session** — so each `wid` follows the *same physical whisker* in every frame — is handled by the linking step in `wwutils/classifiers/hmm_link.py` (`link_whiskers_hmm`): per-side whisk-HMM reclassify → cross-chunk stitch → outlier filters. The HMM linker is solid but hits two ceilings that hand-tuned heuristics can't clear: **coverage** (length/follicle/angle filters drop real, still-visible whiskers) and **close-whisker identity** (adjacent whiskers swap; they separate cleanly by *length*, which the position-keyed linker ignores). The **learned add-on** addresses both: * a **coverage model** — a shipped real-vs-noise classifier (`wwutils/classifiers/models/coverage.joblib`) that replaces the outlier filters and re-admits dropped detections. Real-vs-noise is universal and the features are scale-free, so it is trained once (on two animals, sc013 + seg04) and applied to any clip. Cross-session held-out AP ≈ 0.999. * an **identity re-ranker** — a per-clip model (no labels needed: bootstrapped from the HMM's own high-confidence runs) that uses **length** + normalized follicle position to fix close-whisker swaps via a conservative, no-flicker Hungarian re-rank that only overrides the HMM when its margin is large. ### Day-to-day use The add-on ships with the package and is off by default. To enable it, pass `--learned` to the tracking entry point (`whisker_tracking.py` in the thigmotaxis repo, or call `link_whiskers_hmm(..., coverage_mode="model", identity_mode="bootstrap")` directly): * **Any new clip:** add `--learned`. Zero-shot it already beats the baseline linker (seg04: missing detections 59 → 20, identity accuracy 0.969 → 0.981) — no per-clip setup. * **An important clip you want pristine:** label a few minutes in the GUI, then train a GT-backed identity model for that session — it drives id-switches down hard (seg04 32 → 4). See `identity_model.train_identity(out, gt=...)`. * **Tip:** before labeling/seeking in the GUI, run the clip through a re-encode to an all-intra stream (`ffmpeg -i in.mp4 -c:v libx264 -bf 0 -g 1 out.mp4`) or trace with `--enhance`. Raw H.264 with B-frames over-reports the frame count and seeks inaccurately, so overlays can drift by a frame or two. The add-on is additive and schema-preserving: with the flag off, the linker is byte-identical to before. Coverage is train-once-ship-everywhere; identity is per-session by design. > **Requires** `scikit-learn` and `joblib` (installed automatically as > dependencies). The coverage model is bundled in the wheel.