# DNBelab_C_Series_HT_scRNA-analysis-software
An open source and flexible pipeline to analysis high-throughput DNBelab C Series single-cell RNA datasets
## Introduction
- **Propose**
  - An open source and flexible pipeline to analyze DNBelab C Series<sup>TM</sup> single-cell RNA datasets. 
- **Language**
  - Python3(>=3.8.*),R scripts
- **Hardware/Software requirements** 
  - x86-64 compatible processors.
  - require at least 50GB of RAM and 4 CPU. 
  - centos 7.x 64-bit operating system (Linux kernel 3.10.0, compatible with higher software and hardware configuration). 

## Directory contents
- **DNBC4tools**   config, software and script directories
- **doc**   Instruction for use
- **example** Example of use the pipline
- **scripts**    Miscellaneous scripts
- **wdl**  WDL pipeline

## Installation
installation manual [here](./doc/installation.md)

## Database
Create database manual [here](./doc/database.md)
## Start
**WDL version: Customize the config file, use wdl to analysis**
- **[WDL](./doc/wdl/start.md)**

**Command line version: Using the DNBC4tools command line**
- **[DNBC4tools](./doc/DNBC4tools/start.md)**
