Metadata-Version: 2.4
Name: in-silico-fate-mapping
Version: 0.1.3.1
Summary: Interactive in silico fate mapping from tracking data
Home-page: https://github.com/royerlab/in-silico-fate-mapping
Author: Jordao Bragantini
Author-email: jordao.bragantini@czbiohub.org
License: BSD-3-Clause
Project-URL: Bug Tracker, https://github.com/royerlab/in-silico-fate-mapping/issues
Project-URL: Documentation, https://github.com/royerlab/in-silico-fate-mapping#README.md
Project-URL: Source Code, https://github.com/royerlab/in-silico-fate-mapping
Project-URL: User Support, https://github.com/royerlab/in-silico-fate-mapping/issues
Classifier: Development Status :: 2 - Pre-Alpha
Classifier: Framework :: napari
Classifier: Intended Audience :: Developers
Classifier: License :: OSI Approved :: BSD License
Classifier: Operating System :: OS Independent
Classifier: Programming Language :: Python
Classifier: Programming Language :: Python :: 3
Classifier: Programming Language :: Python :: 3 :: Only
Classifier: Programming Language :: Python :: 3.8
Classifier: Programming Language :: Python :: 3.9
Classifier: Programming Language :: Python :: 3.10
Classifier: Topic :: Scientific/Engineering :: Image Processing
Requires-Python: >=3.8
Description-Content-Type: text/markdown
License-File: LICENSE
Requires-Dist: numpy
Requires-Dist: pandas
Requires-Dist: scikit-learn
Requires-Dist: zarr
Requires-Dist: magicgui
Requires-Dist: qtpy
Requires-Dist: napari
Requires-Dist: click
Provides-Extra: testing
Requires-Dist: tox; extra == "testing"
Requires-Dist: pytest; extra == "testing"
Requires-Dist: pytest-cov; extra == "testing"
Requires-Dist: pytest-qt; extra == "testing"
Requires-Dist: pyqt5; extra == "testing"
Dynamic: license-file

# in silico fate mapping

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Interactive in silico fate mapping from tracking data.

This napari plugin estimates the cell fates from tracking data by building a radial regression model per time point. The user can select an area of interest using a `Points` layer; the algorithm will advent the probed coordinates forward (or backward) in time, showing the estimated fate.

Video example below:

https://user-images.githubusercontent.com/21022743/216478216-89c1c35f-2ce4-44e8-adb8-9aeea75b5833.mp4

## Installation

We suggest you create a fresh conda environment to avoid conflicts with your existing package.
To do this, you need to:

    conda create -n fatemap python=3.11
    conda activate fatemap

And then, you can install `in-silico-fate-mapping` via [pip] and other additional useful packages:

    pip install napari-ome-zarr napari[all] in-silico-fate-mapping

To install the latest development version :

    pip install git+https://github.com/royerlab/in-silico-fate-mapping.git


## IO file format

This plugin does not depend on a specific file format, the only requirement is using a `Track` layer from napari.

Despite this, we ship a reader and writer interface. It supports `.csv` files with the following reader `track_id, t, (z), y, x`, `z` is optional.
Such that each tracklet has a unique `track_id` and it's composed of a sequence o time and spatial coordinates.

This is extremely similar to how napari store tracks, more information can be found [here](https://napari.org/stable/howtos/layers/tracks.html).

Divisions are not supported at the moment.

## Usage Example

### Minimal example

Minimal example using a track file following the convention described above.

```python3
import napari
import pandas as pd
from in_silico_fate_mapping.fate_mapping import FateMapping

tracks = pd.read_csv("tracks.csv")

fate_map = FateMapping(radius=5, n_samples=25, bind_to_existing=False, sigma=1)
fate_map.data = tracks[["track_id", "t", "z", "y", "x"]]

source = tracks[tracks["t"] == 0].sample(n=1)

tracks = fate_map(source[["t", "z", "y", "x"]])

napari.view_tracks(tracks)
napari.run()
```

### Zebrahub example

Zebrafish embryo tail example. This example requires the package `napari-ome-zarr`.

```python3
import napari
import pandas as pd
from in_silico_fate_mapping import FateMappingWidget

image_path = "http://public.czbiohub.org/royerlab/zebrahub/imaging/single-objective/ZSNS001_tail.ome.zarr"
tracks_path = "http://public.czbiohub.org/royerlab/zebrahub/imaging/single-objective/ZSNS001_tail_tracks.csv"

viewer = napari.Viewer()
viewer.window.add_dock_widget(FateMappingWidget(viewer))

viewer.open(image_path, plugin="napari-ome-zarr")

tracks = pd.read_csv(tracks_path)
viewer.add_tracks(tracks[["track_id", "t", "z", "y", "x"]])
viewer.add_points(name="Markers", ndim=4)

napari.run()
```

## Citing

If used please cite:

```
@article{lange2023zebrahub,
  title={Zebrahub-Multimodal Zebrafish Developmental Atlas Reveals the State Transition Dynamics of Late Vertebrate Pluripotent Axial Progenitors},
  author={Lange, Merlin and Granados, Alejandro and VijayKumar, Shruthi and Bragantini, Jordao and Ancheta, Sarah and Santhosh, Sreejith and Borja, Michael and Kobayashi, Hirofumi and McGeever, Erin and Solak, Ahmet Can and others},
  journal={bioRxiv},
  pages={2023--03},
  year={2023},
  publisher={Cold Spring Harbor Laboratory}
}
```

## Issues

If you encounter any problems, please [file an issue] along with a detailed description.

[napari]: https://github.com/napari/napari
[Cookiecutter]: https://github.com/audreyr/cookiecutter
[@napari]: https://github.com/napari
[MIT]: http://opensource.org/licenses/MIT
[BSD-3]: http://opensource.org/licenses/BSD-3-Clause
[GNU GPL v3.0]: http://www.gnu.org/licenses/gpl-3.0.txt
[GNU LGPL v3.0]: http://www.gnu.org/licenses/lgpl-3.0.txt
[Apache Software License 2.0]: http://www.apache.org/licenses/LICENSE-2.0
[Mozilla Public License 2.0]: https://www.mozilla.org/media/MPL/2.0/index.txt
[cookiecutter-napari-plugin]: https://github.com/napari/cookiecutter-napari-plugin

[file an issue]: https://github.com/royerlab/in-silico-fate-mapping/issues

[napari]: https://github.com/napari/napari
[tox]: https://tox.readthedocs.io/en/latest/
[pip]: https://pypi.org/project/pip/
[PyPI]: https://pypi.org/
